Anti-CD64 抗体 [EPR4623] - Low endotoxin, Azide free (ab246692)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4623] to CD64 - Low endotoxin, Azide free
- Suitable for: WB, IP, Flow Cyt
- Knockout validated
- Reacts with: Human
製品の概要
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製品名
Anti-CD64 antibody [EPR4623] - Low endotoxin, Azide free
CD64 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4623] to CD64 - Low endotoxin, Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, Flow Cytmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- U937, THP1, and HL60 cell lysates; U937 cells.
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特記事項
ab246692 is the carrier-free version of ab109449.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.00 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Endotoxin level is less than 1 EU/ml as determined by the TAL test. -
ポリ/モノ
モノクローナル -
クローン名
EPR4623 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-CD64 antibody [EPR4623] (ab109449)
- Anti-CD64 antibody [EPR4623] - BSA and Azide free (ab216856)
- Alexa Fluor® 488 Anti-CD64 antibody [EPR4623] (ab309949)
- Alexa Fluor® 647 Anti-CD64 antibody [EPR4623] (ab310316)
- Alexa Fluor® 594 Anti-CD64 antibody [EPR4623] (ab310780)
- Alexa Fluor® 555 Anti-CD64 antibody [EPR4623] (ab312306)
- Alexa Fluor® 568 Anti-CD64 antibody [EPR4623] (ab312802)
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab246692の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa). |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. -
組織特異性
Monocyte/macrophage specific. -
配列類似性
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Phosphorylated on serine residues. -
細胞内局在
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. - Information by UniProt
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参照データベース
- Entrez Gene: 2209 Human
- Entrez Gene: 2210 Human
- Omim: 146760 Human
- Omim: 601502 Human
- Omim: 601503 Human
- SwissProt: A6NKC4 Human
- SwissProt: P12314 Human
- SwissProt: Q92637 Human
see all -
別名
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
see all
画像
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All lanes : Anti-CD64 antibody [EPR4623] (ab109449) at 1/1000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : FCGR1A knockout THP-1 cell lysate
Lane 3 : U937 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD64 antibody [EPR4623] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109449 was shown to bind specifically to CD64. A band was observed at 45-50/55-80 kDa in wild-type THP-1 cell lysates with no signal observed at this size in FCGR1A knockout cell line ab275843 (knockout cell lysate ab275817). To generate this image, wild-type and FCGR1A knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109449).
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109449).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab246692 は論文での使用が確認できていません。