製品の概要
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製品名Anti-CD63 antibody [NK1/C3]
CD63 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [NK1/C3] to CD63
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由来種Mouse
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特異性This antibody is specific to a 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells.
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アプリケーション適用あり: ICC, IHC-P, IHC-Fr, Flow Cytmore details
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種交差性交差種: Human
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免疫原
Smooth plasma membrane fraction of MeWo cells.
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特記事項
CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed in granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen ME491 and to the platelet antigen PTLGP40.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium Azide
Constituents: 1% BSA -
Concentration information loading... -
精製度IgG fraction
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一次抗体 備考CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed in granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen ME491 and to the platelet antigen PTLGP40.
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ポリ/モノモノクローナル
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クローン名NK1/C3
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アイソタイプIgG1
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| ICC | Use at an assay dependent concentration. | |
| IHC-P | 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | 1/100. (Also see PMID 19129916).
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
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組織特異性Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
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配列類似性Belongs to the tetraspanin (TM4SF) family.
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細胞内局在Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
- Information by UniProt
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参照データベース
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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別名
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NK1/C3] (ab1318)](https://www.abcam.co.jp/ps/products/1/ab1318/Images/ab1318_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NK1/C3] (ab1318)ab1318 staining human melanoma by IHC-P. -
![Flow Cytometry - Anti-CD63 antibody [NK1/C3] (ab1318)](https://www.abcam.co.jp/ps/products/1/ab1318/Images/CD63-Primary-antibodies-ab1318-3.jpg)
Flow Cytometry - Anti-CD63 antibody [NK1/C3] (ab1318)Overlay histogram showing HL60 cells stained with ab1318 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1318, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
参考文献
This product has been referenced in:
- Aaberg-Jessen C et al. Co-expression of TIMP-1 and its cell surface binding partner CD63 in glioblastomas. BMC Cancer 18:270 (2018). Read more (PubMed: 29523123) »
- Khazen R et al. Melanoma cell lysosome secretory burst neutralizes the CTL-mediated cytotoxicity at the lytic synapse. Nat Commun 7:10823 (2016). Read more (PubMed: 26940455) »
レビューと Q&A
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