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  1. Link

    cd63-antibody-mem-259-ab8219.pdf

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Cardiovascular Blood Platelets
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Anti-CD63 抗体 [MEM-259] (ab8219)

  • Datasheet
  • SDS
Reviews (8)Q&A (13)References (81)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (ab8219)
  • Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)
  • Flow Cytometry - Anti-CD63 antibody [MEM-259] (ab8219)
  • Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)
  • Flow Cytometry - Anti-CD63 antibody [MEM-259] (ab8219)
  • Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)

Key features and details

  • Mouse monoclonal [MEM-259] to CD63
  • Suitable for: Flow Cyt, ICC, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Biotin FITC PE PE/Cy5® PerCP

こちらの製品もご検討ください

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Anti-CD63 antibody [MX-49.129.5] - BSA and Azide free (ab213092)
一次抗体
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Anti-CD81 antibody [1D6] (ab23505)

関連製品

製品の概要

  • 製品名

    Anti-CD63 antibody [MEM-259]
    CD63 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [MEM-259] to CD63
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Flow Cyt, ICC, IHC-Pmore details
    適用なし: WB
  • 種交差性

    交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human CD63. HPB-ALL T cell line

  • ポジティブ・コントロール

    • IHC: Human spleen sections; ICC: Human HeLa cells, primary fibroblasts and skin fibroblasts tissues; Flow cyt: Human blood samples.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    MEM-259
  • アイソタイプ

    IgG1
  • 研究分野

    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

関連製品

  • Alternative Versions

    • Biotin Anti-CD63 antibody [MEM-259] (ab134331)
    • FITC Anti-CD63 antibody [MEM-259] (ab18235)
    • PE Anti-CD63 antibody [MEM-259], prediluted (ab18236)
    • PE/Cy5® Anti-CD63 antibody [MEM-259] (ab234251)
    • PerCP Anti-CD63 antibody [MEM-259] (ab77227)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8219の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (2)
Use a concentration of 2 µg/ml.

Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC (1)
Use at an assay dependent concentration.
IHC-P (1)
Use a concentration of 10 µg/ml.
特記事項
Flow Cyt
Use a concentration of 2 µg/ml.

Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC
Use at an assay dependent concentration.
IHC-P
Use a concentration of 10 µg/ml.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • 組織特異性

    Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • 配列類似性

    Belongs to the tetraspanin (TM4SF) family.
  • 細胞内局在

    Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Target information above from: UniProt accession P08962 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 967 Human
    • Omim: 155740 Human
    • SwissProt: P08962 Human
    • Unigene: 445570 Human
    • 別名

      • Lysosomal associated membrane protein 3 antibody
      • CD 63 antibody
      • CD63 antibody
      • CD63 antigen (melanoma 1 antigen) antibody
      • CD63 antigen antibody
      • CD63 antigen melanoma 1 antigen antibody
      • CD63 molecule antibody
      • CD63_HUMAN antibody
      • gp55 antibody
      • Granulophysin antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • LAMP3 antibody
      • LIMP antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome associated membrane glycoprotein 3 antibody
      • Lysosome-associated membrane glycoprotein 3 antibody
      • Mast cell antigen AD1 antibody
      • ME491 antibody
      • Melanoma 1 antigen antibody
      • Melanoma associated antigen ME491 antibody
      • Melanoma associated antigen MLA1 antibody
      • Melanoma-associated antigen ME491 antibody
      • MGC72893 antibody
      • MLA 1 antibody
      • MLA1 antibody
      • NGA antibody
      • Ocular melanoma associated antigen antibody
      • Ocular melanoma-associated antigen antibody
      • OMA81H antibody
      • PTLGP40 antibody
      • Tetraspanin 30 antibody
      • Tetraspanin-30 antibody
      • Tspan 30 antibody
      • Tspan-30 antibody
      • TSPAN30 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (ab8219)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (ab8219)

      Immunohistochemistry staining of human spleen (paraffin sections) using ab8219.

    • Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)
      Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)

      Immunocytochemistry staining of human skin fibroblasts with ab8219 (green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

    • Flow Cytometry - Anti-CD63 antibody [MEM-259] (ab8219)
      Flow Cytometry - Anti-CD63 antibody [MEM-259] (ab8219)This image is courtesy of an anonymous Abreview
      ab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)
      Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)

      Immunocytochemistry staining of CD63 in human HeLa cell line using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

    • Flow Cytometry - Anti-CD63 antibody [MEM-259] (ab8219)
      Flow Cytometry - Anti-CD63 antibody [MEM-259] (ab8219)

      Flow cytometry analysis staining CD63 in IgE- activated peripheral blood using ab8219 (2μg/ml). 

    • Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)
      Immunocytochemistry - Anti-CD63 antibody [MEM-259] (ab8219)

      Immunocytochemistry staining of CD63 in human primary fibroblasts using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

    プロトコール

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (81)

    ab8219 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8219 は 81 報の論文で使用されています。

    • Ji D  et al. Small-sized extracellular vesicles (EVs) derived from acute myeloid leukemia bone marrow mesenchymal stem cells transfer miR-26a-5p to promote acute myeloid leukemia cell proliferation, migration, and invasion. Hum Cell 34:965-976 (2021). PubMed: 33620671
    • Ivanusic D  et al. The large extracellular loop of CD63 interacts with gp41 of HIV-1 and is essential for establishing the virological synapse. Sci Rep 11:10011 (2021). PubMed: 33976357
    • Yamamoto S  et al. Specimen-specific drift of densities defines distinct subclasses of extracellular vesicles from human whole saliva. PLoS One 16:e0249526 (2021). PubMed: 33831057
    • Wang Y  et al. miR-224-5p Carried by Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes Regulates Autophagy in Breast Cancer Cells via HOXA5. Front Cell Dev Biol 9:679185 (2021). PubMed: 34095151
    • Li J  et al. Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes. PLoS One 15:e0239153 (2020). PubMed: 32941510
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 21 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-CD63 antibody [MEM-259]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Melanoma cells)
    Permeabilization
    No
    Specification
    Melanoma cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 05 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD63 antibody [MEM-259]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Melanoma cell)
    Permeabilization
    No
    Specification
    Melanoma cell
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 May 26 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-CD63 antibody [MEM-259]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Melanoma)
    Permeabilization
    No
    Specification
    Melanoma
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 May 19 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD63 antibody [MEM-259]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Melanoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Specification
    Melanoma
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 07 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD63 antibody [MEM-259]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Cell (Blood film)
    Specification
    Blood film
    Permeabilization
    Yes - 0.1M PBS with 1% TritonX100
    Fixative
    Paraformaldehyde
    Read More

    DR. Ruma Raha-Chowdhury

    Verified customer

    投稿 Sep 26 2014

    Question


    I have a question for 2 of your products an anti-CD9 (ab2215) and an anti-CD63 (ab8219).
    There is an information in their product Datsheet that I don't really understand.
    Ex : For WB use a concentration of xx µg/ml. Use under non reducing condition
    "Non reducing condition" is about my sample? These antibodies need dissulfide bound to recognize epitopes?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 27 2013

    Answer

    ab2215 should be used at a concentration of 2-4 ug/ml in blocking buffer when incubating your membrane whereas ab8216 should be used at a concentration of 2 ug/ml.

    The gel should be run in non-reducing conditions as these antibodies do not recognise their antigens well when the western blot sample contains reducing agent. We therefore advise against adding beta-mercaptoethanol or DTT in the loading buffer.

    Read More

    Abcam Scientific Support

    Answered on Mar 27 2013

    Question

    I have a question about stability of anti-CD63 antibody [MEM-259; ab8219) under alkaline environment.
    I need to run experiments to immobilize antibody chemically at pH 9. Will the antibody be stable at pH 9 (for 2hr)?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 10 2013

    Answer


    We have not tested the stability of ab8219 under the alkaline conditions that you described; thus, we cannot guarantee that it will work under these conditions. The laboratory thinks it is possible that it could work, but only performing the experiment will tell.

    Read More

    Abcam Scientific Support

    Answered on Jan 10 2013

    Question

    Please send a reference describing characterization of this clone. Has the epitope been mapped?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 18 2012

    Answer

    We are not aware of a reference describing production and characterization of this CD63 antibody. The epitope the antibody recognizes on CD63 has not, to our knowledge, been mapped, but we do know that the epitope is extracellular when CD63 is integrated into the plasma membrane.

    Read More

    Abcam Scientific Support

    Answered on Dec 18 2012

    Question

    I have recently purchased two primary antibodies, anti-CD9 antibody (ab2215) and anti-CD63 (ab8219) which I have been using with human proteins. It has been working fine, but I was wondering if you've encountered binding with Bovine proteins.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2012

    Answer

    Thank you for contacting us.

    The antibodies have not been tested for reactivity with bovine samples, and we have not received reports of bovine reactivity from our customers.

    Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 18 2012

    Question

    I have just started my antibody optimization for a CD63 antibody (ab8219). Could you tell me the volume of antibody sent out, its concentration, and the final concentration needed when incubating in the primary antibody, ie the ideal dilution needed for this antibody. Thanks for your help!

    Read More

    Abcam community

    Verified customer

    Asked on Jun 22 2012

    Answer

    Thank you for your inquiry.

    Ab8219 (anti-CD63) is supplied as 100ul at a concentration of 1mg/ml. We have a customer who reported successfully using the antibody at a 1/200 dilution (0.2 mg/ml), but it may be necessary to optimize this for your own experiments. Please note that we recommend using non-reducing conditions for the western blot (i.e. no BME or DTT).

    I hope this information helps. Please do not hesitate to contact us if you need anything further. Have a wonderful day, and best of luck in your research!

    Read More

    Abcam Scientific Support

    Answered on Jun 22 2012

    1-10 of 21 Abreviews or Q&A

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