Anti-CD63 抗体 [KILL150A] (ab271286)
![Western blot - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.co.jp/ps/products/271/ab271286/Images/ab271286-395330-anticd63-antibody-kill150a-western-blot-skmel28.jpg "Western blot - Anti-CD63 antibody [KILL150A] (ab271286)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [KILL150A] to CD63
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
製品の概要
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製品名
Anti-CD63 antibody [KILL150A]
CD63 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [KILL150A] to CD63 -
由来種
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: SK-MEL-28 and HUVEC whole cell lysate. Human lymph node tissue lysate. IHC-P: Human melanoma tissue. ICC/IF: SK-MEL-28 cells. Flow Cyt: SK-MEL-28
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
KILL150A -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
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| IHC-P |
Human
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| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.978 µg/ml. Predicted molecular weight: 26 kDa.
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| IHC-P | (1) |
Use a concentration of 0.978 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Flow Cyt |
Use a concentration of 19.56 µg/ml.
|
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| ICC/IF |
Use a concentration of 19.56 µg/ml.
|
| 特記事項 |
|---|
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WB
Use a concentration of 0.978 µg/ml. Predicted molecular weight: 26 kDa. |
|
IHC-P
Use a concentration of 0.978 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt
Use a concentration of 19.56 µg/ml. |
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ICC/IF
Use a concentration of 19.56 µg/ml. |
ターゲット情報
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機能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
組織特異性
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
細胞内局在
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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参照データベース
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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別名
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
画像
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Western blot - Anti-CD63 antibody [KILL150A] (ab271286)All lanes : Anti-CD63 antibody [KILL150A] (ab271286) at 0.978 µg/ml
Lane 1 : SK-MEL-28 (human malignant melanoma), whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 3 : Human lymph node tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 26 kDaCD63 can undergo glycosylation as shown in lane 1, 2 and 3 (PMID: 9890706, 28740179).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 3 mins; Lane 3: 48 secs.
This blot was developed using a higher sensitivity ECL substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.co.jp/ps/products/271/ab271286/Images/ab271286-1-anticd63-antibody-kill150a-immunohistochemistry-human-melanoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] (ab271286)Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling CD63 with ab271286 at 1/4000 dilution (0.978µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human melanoma. The section was incubated with ab271268 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.co.jp/ps/products/271/ab271286/Images/ab271286-2-anticd63-antibody-kill150a-immunohistochemistry-human-breast.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] (ab271286)Immunohistochemical analysis of paraffin-embedded human breast tissue labeling CD63 with ab271286 at 1/4000 dilution (0.978µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab271268 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: Nearly no staining on human breast.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.co.jp/ps/products/271/ab271286/Images/ab271286-3-anticd63-antibody-kill150a-immunocytochemistry-skmel28.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [KILL150A] (ab271286)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 cells labelling CD63 with ab271286 at 1/50 dilution (19.56µg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing lysosome/endosomes (ab252919) co-staining in SK-MEL-28 cells.
ab252919 Anti-CD63 rabbit monoclonal antibody was used to counterstain tubulin at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab271286 at a 1/50 dilution (19.56µg/ml) followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab259919 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Flow Cytometry - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.co.jp/ps/products/271/ab271286/Images/ab271286-5-anticd63-antibody-kill150a-flow-cytometry-skmel28.jpg)
Flow Cytometry - Anti-CD63 antibody [KILL150A] (ab271286)Flow cytometric analysis of SK-MEL-28 (human malignant melanoma) cells labelling CD63 with ab271286 at 1/1000 dilution (19.56µg/ml) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.co.jp/ps/products/271/ab271286/Images/ab271286-7-benefits-of-recombinant-antibodies.png)
Anti-CD63 antibody [KILL150A] (ab271286)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271286 は論文での使用が確認できていません。