Anti-CD63 抗体 (ab231975)
Key features and details
- Rabbit polyclonal to CD63
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-CD63 antibody
CD63 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD63 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human, Pig -
免疫原
Recombinant fragment corresponding to Human CD63 aa 100-250. N-terminal His and GST tags, Expressed in E.coli.
Database link: P08962 -
ポジティブ・コントロール
- WB: Recombinant human CD63 protein; Human serum; Pig liver lysate; A-375 cell lysate. IHC-P: Human colon cancer, stomach, prostate adenoma, skin cancer, breast cancer and liver tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab231975の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa.
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特記事項 |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa. |
ターゲット情報
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機能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
組織特異性
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
細胞内局在
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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参照データベース
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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別名
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
画像
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All lanes : Anti-CD63 antibody (ab231975) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : Pig liver lysate
Lane 3 : A375 (human malignant melanoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
Anti-CD63 antibody (ab231975) at 2 µg/ml + Recombinant human CD63 protein
Predicted band size: 26 kDa -
Formalin-fixed, paraffin-embedded human liver tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human skin cancer tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate adenoma tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab231975 は 5 報の論文で使用されています。
- Lai C et al. Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis rats. Mol Cell Biochem 478:637-649 (2023). PubMed: 36001206
- Northrop-Albrecht EJ et al. Assessment of extracellular vesicle isolation methods from human stool supernatant. J Extracell Vesicles 11:e12208 (2022). PubMed: 35383410
- Lucien F et al. Poly (ADP-Ribose) and α-synuclein extracellular vesicles in patients with Parkinson disease: A possible biomarker of disease severity. PLoS One 17:e0264446 (2022). PubMed: 35395000
- Li B et al. Synchronous Disintegration of Ferroptosis Defense Axis via Engineered Exosome-Conjugated Magnetic Nanoparticles for Glioblastoma Therapy. Adv Sci (Weinh) 9:e2105451 (2022). PubMed: 35508804
- Hua R et al. Small RNA-seq analysis of extracellular vesicles from porcine uterine flushing fluids during peri-implantation. Gene 766:145117 (2021). PubMed: 32920039