Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 10% rabbit serum in PBS
Secondary antibody
Name
Dilution
1/100
Additional data
Additional Notes
Image shows upregulation of E-selectin in microvessels of IL-1 injected mouse brain. The secondary antibody was biotinylated, followed by incubation with a HRP-streptavidin conjugate (VectorABC, Vectorlabs) and DAB staining (brown colour). Counterstain: Chresyl violet.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Thomas Weissensteiner
Verified customer
投稿 Oct 22 2009