製品の概要

  • 製品名
    Anti-CD62E antibody [UZ6]
    CD62E 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [UZ6] to CD62E
  • 由来種
    Rat
  • アプリケーション
    適用あり: ICC/IF, ELISA, Flow Cyt, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Mouse
  • ポジティブ･コントロール
    • Tonsil

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 67 kDa.

ターゲット情報

  • 機能
    Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • 配列類似性
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • CD26E antibody
      • CD62 antigen-like family member E antibody
      • CD62E antibody
      • E selectin antibody
      • E-selectin antibody
      • ELAM antibody
      • ELAM-1 antibody
      • ELAM1 antibody
      • Endothelial adhesion molecule 1 antibody
      • Endothelial leukocyte adhesion molecule 1 antibody
      • ESEL antibody
      • LECAM2 antibody
      • Leukocyte-endothelial cell adhesion molecule 2 antibody
      • LYAM2_HUMAN antibody
      • Sele antibody
      • Selectin E antibody
      see all

    参考文献

    This product has been referenced in:
    • Sapieha P  et al. 5-Lipoxygenase metabolite 4-HDHA is a mediator of the antiangiogenic effect of ?-3 polyunsaturated fatty acids. Sci Transl Med 3:69ra12 (2011). Read more (PubMed: 21307302) »
    • Hammel M  et al. Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion. Exp Cell Res 269:266-74 (2001). Read more (PubMed: 11570819) »

    See all 2 Publications for this product

    ab2497 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    4 reviews or Q&A

    10
    Votes: Highest First

    Thank you so much for your email. I would like to ask you a question.

    I have to target 3 proteins in my Mouse Brain Enothelial cell line (bEnd.3), which are E-selectin (CD62E), VCAM and ICAM.

    I have chosen ab2497 for E-selectin , ...

    Read More

    Thanks you for your email.
    In my previous email there is a correction about the expiry date. The expiry date should be read as 25-08-2012.
    I have asked lab to release the immunogen information of these antibodies; I will be able to send the a...

    Read More

    Hi Technical Support, how are you today?

    Could you please advise me on the difference between the corresponding pairs below:

    ab 22631 Anti-CD62E antibody

    &

    ab2497 Anti-CD62E antibody

    and
    ...

    Read More

    Thank you very much for your email!

    I hope you are well as well! Here we are fine - we have one of our first days of spring :-)

    In regards to your questions:

    The CD62E antibodies ab22631 et ab2497 are two different clones....

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-CD62E antibody [UZ6]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    abreview image
    Username

    Dr. Thomas Weissensteiner

    Verified customer

    投稿 Oct 22 2009

    1) Crossreactivities? 2) Is it function blocking or not?

    1) Crossreactivities: this antibody reacts with mouse E-selectin but not with human E-selectin. Other species not tested. 2)Blocking function: Ab2497 (20 microgram/ml) inhibited up to 70% of mouse PMN and up to 95% of human peripheral blood mononuclea...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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