Application
ELISA
Sample
Human Cell (HUVEC)
Specification
HUVEC
Type
Direct
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG HRP cell signal #7076
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Additional data
Additional Notes
This antibody does not work on PFA fixed.
Abcam response
Our scientific support team are currently investigating the PFA fixed IHC results with the customer.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 07 2011