製品名Anti-CD6 / T12 antibody [EPR4056]
CD6 / T12 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4056] to CD6 / T12
アプリケーション適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt or ICC
Synthetic peptide within Human CD6/ T12 aa 1-100. The exact sequence is proprietary.
- WB: Jurkat, HuT-78, PBMC and Human tonsil lysates IHC-P: Human tonsil and Human lymphocytes infiltrating colon tissues
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as CD6.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab109218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 72 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Antigen retrieval is recommended.|
機能Involved in cell adhesion. Binds to CD166.
組織特異性Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.
配列類似性Contains 3 SRCR domains.
翻訳後修飾After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
Contains intrachain disulfide bond(s).
- Information by UniProt
- CD_antigen=CD6 antibody
- CD6 antibody
- CD6 antigen Tp120 antibody
All lanes : Anti-CD6 / T12 antibody [EPR4056] (ab109218) at 1/1000 dilution
Lane 1 : Jurkat lysates
Lane 2 : HuT-78 lysates
Lane 3 : PBMC lysates
Lane 4 : Human tonsil lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 90-130 kDa why is the actual band size different from the predicted?
ab109218, at a 1/100 dilution, staining CD6 / T12 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
ab109218, at a 1/100 dilution, staining CD6 / T12 in paraffin embedded Human lymphocytes infiltrating colon tissue by Immunohistochemistry.
ab109218 showing negative staining in Normal kidney tissue.
ab109218 showing negative staining in Normal uterus tissue.
ab109218 showing negative staining in Normal brain tissue.
ab109218 has not yet been referenced specifically in any publications.