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    cd59-antibody-mem-43-ab9182.pdf

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Immunology Cell Type Markers CD Non-lineage
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Anti-CD59 抗体 [MEM-43] (ab9182)

  • Datasheet
  • SDS
Reviews (2)Q&A (9)References (22)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182)

    Key features and details

    • Mouse monoclonal [MEM-43] to CD59
    • Suitable for: IP, IHC-Fr, IHC, ICC/IF, Flow Cyt, WB, IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 647 APC Biotin FITC PE

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    一次抗体
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    Anti-CD59 antibody [EPR6425(2)] (ab133707)
    タンパク質
    Product image
    Recombinant Human CD59 protein (ab134887)

    関連製品

    製品の概要

    • 製品名

      Anti-CD59 antibody [MEM-43]
      CD59 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [MEM-43] to CD59
    • 由来種

      Mouse
    • 特異性

      CD59 antigen (human). MEM-43 identified CD59 as the new cluster on 4th HLDA Workshop. MEM-43 reacts with well defined epitope (W40, R53).

    • アプリケーション

      適用あり: IP, IHC-Fr, IHC, ICC/IF, Flow Cyt, WB, IHC-Pmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Tissue, cells or virus corresponding to Human CD59. Thymocytes and T lymphocytes

    • ポジティブ・コントロール

      • ICC/IF: Human fibrosarcoma cells. Flow Cyt: HT1080 cells. IHC: human placenta tissue
    • 特記事項

      When originally tested in WB, SDS was included in the sample buffer, however, feedback from one researcher has shown that it is best to omit SDS from the sample buffer.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Do Not Freeze.
    • バッファー

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Protein A purified
    • 特記事項(精製)

      Purity >95% by SDS-PAGE.
    • ポリ/モノ

      モノクローナル
    • クローン名

      MEM-43
    • ミエローマ

      unknown
    • アイソタイプ

      IgG2a
    • 軽鎖の種類

      unknown
    • 研究分野

      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Immunology
      • Innate Immunity
      • Complement
      • MAC
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Other
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity

    関連製品

    • Alternative Versions

      • FITC Anti-CD59 antibody [MEM-43], prediluted (ab18237)
      • Alexa Fluor® 647 Anti-CD59 antibody [MEM-43], prediluted (ab187769)
      • Biotin Anti-CD59 antibody [MEM-43] (ab28114)
      • PE Anti-CD59 antibody [MEM-43] (ab36421)
      • APC Anti-CD59 antibody [MEM-43] (ab36467)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human CD59 protein (ab134887)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9182の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IP
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC
    Use a concentration of 10 µg/ml.
    ICC/IF (1)
    Use at an assay dependent concentration. PubMed: 17911601
    Flow Cyt (1)
    Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    WB
    Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

    Use under non-reducing conditions.

    IHC-P
    Use at an assay dependent concentration.
    特記事項
    IP
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC
    Use a concentration of 10 µg/ml.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 17911601
    Flow Cyt
    Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    WB
    Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

    Use under non-reducing conditions.

    IHC-P
    Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
      The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
    • 関連疾患

      Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
    • 配列類似性

      Contains 1 UPAR/Ly6 domain.
    • 翻訳後修飾

      N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
      Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
    • 細胞内局在

      Cell membrane. Secreted. Soluble form found in a number of tissues.
    • Target information above from: UniProt accession P13987 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 966 Human
      • Omim: 107271 Human
      • SwissProt: P13987 Human
      • Unigene: 278573 Human
      • Unigene: 709466 Human
      • Unigene: 710641 Human
      • 別名

        • 16.3A5 antibody
        • 1F5 antibody
        • 1F5 antigen antibody
        • 20 kDa homologous restriction factor antibody
        • CD 59 antibody
        • CD_antigen=CD59 antibody
        • CD59 antibody
        • CD59 antigen antibody
        • CD59 antigen complement regulatory protein antibody
        • CD59 antigen p18 20 antibody
        • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
        • CD59 glycoprotein antibody
        • CD59 molecule antibody
        • CD59 molecule complement regulatory protein antibody
        • CD59_HUMAN antibody
        • Cd59a antibody
        • Complement regulatory protein antibody
        • EJ16 antibody
        • EJ30 antibody
        • EL32 antibody
        • FLJ38134 antibody
        • FLJ92039 antibody
        • G344 antibody
        • HRF 20 antibody
        • HRF-20 antibody
        • HRF20 antibody
        • Human leukocyte antigen MIC11 antibody
        • Ly 6 like protein antibody
        • Lymphocytic antigen CD59/MEM43 antibody
        • MAC inhibitory protein antibody
        • MAC IP antibody
        • MAC-inhibitory protein antibody
        • MAC-IP antibody
        • MACIF antibody
        • MACIP antibody
        • MEM43 antibody
        • MEM43 antigen antibody
        • Membrane attack complex (MAC) inhibition factor antibody
        • Membrane attack complex inhibition factor antibody
        • Membrane inhibitor of reactive lysis antibody
        • MGC2354 antibody
        • MIC11 antibody
        • MIN1 antibody
        • MIN2 antibody
        • MIN3 antibody
        • MIRL antibody
        • MSK21 antibody
        • p18 20 antibody
        • Protectin antibody
        • Surface antigen recognized by monoclonal antibody 16.3A5 antibody
        • T cell activating protein antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182)

        Immunohistochemistry parafin embedded sections staining of huam palacenta tissue using ab9182 with a concentration of 10µg / ml

      プロトコール

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (22)

      ab9182 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab9182 は 22 報の論文で使用されています。

      • Brandel A  et al. The Gb3-enriched CD59/flotillin plasma membrane domain regulates host cell invasion by Pseudomonas aeruginosa. Cell Mol Life Sci 78:3637-3656 (2021). PubMed: 33555391
      • Yan B  et al. Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression. Biomed Res Int 2020:2753414 (2020). PubMed: 32337233
      • Kalappurakkal JM  et al. Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading. Cell 177:1738-1756.e23 (2019). PubMed: 31104842
      • Yan B  et al. iTRAQ-based Comparative Serum Proteomic Analysis of Prostate Cancer Patients with or without Bone Metastasis. J Cancer 10:4165-4177 (2019). PubMed: 31413735
      • Chen Y  et al. Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds. J Cell Mol Med 23:5956-5969 (2019). PubMed: 31293077
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 11 Abreviews or Q&A

      Flow Cytometry abreview for Anti-CD59 antibody [MEM-43]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (Fibrosarcoma (HT1080))
      Specification
      Fibrosarcoma (HT1080)
      Fixation
      Paraformaldehyde
      Permeabilization
      No
      Gating Strategy
      no gating
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 13 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD59 antibody [MEM-43]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (fibrosarcoma)
      Specification
      fibrosarcoma
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 19 2008

      Question

      I purchased ab9182 which is CD59. I have try dilutions from 1/10 to 1/100 and nothing seems to work. I don't get any staining. Today I tried with placenta, 1/20, antigen retrieval with Tris for 32 min and again nothing. I'm using Benchmark automated stainer. Any suggestions??? The lot # is GR36289-3 and order #1021675. Let me know if you need any other information.
      Thanks,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 21 2012

      Answer

      Thank you for contacting us. I am sorry to hear that you are having trouble using this antibody.

      I have read the information you have kindly provided and would like to ask few questions for better understanding of the problem;

      - Could you explain the antigen retrieval method bit more? The information I am after is Buffer, buffer ph; hot plate boiling method, microwave boiling method or pressure cooker method.
      - Are you suing human placenta sections?
      - Which statement you would choose e.g. high background, no staining or light staining.
      - What was the secondary antibody e.g species, type, isotype, dilution etc?
      - Have you used same secondary antibody before? Is it true the anomaly is not due to secondary antibody?
      - Does the detection system works fine? Have you tried using it with other antibodies and tissue sections?
      - Could you send us an image?
      - How the antibody was stored since ordering?
      - Have you preformed any other troubleshooting than changing dilutions?

      In order to help you further with this antibody I will look forward to hearing from you .

      Read More

      Abcam Scientific Support

      Answered on Mar 21 2012

      Question


      This is in regards to our Mouse monoclonal [MEM-43] to CD59, which the datasheet says is valid for Functional Elisa, but says it contains Azide, which is confusing to the customer.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 15 2013

      Answer


      We have removed functional studies from the datasheet for ab9182 since the sodium azide present in the storage buffer is toxic to cells.

      Alternatively, we have a sodium azide free anti-CD59 antibody that has been tested in functional studies:
      https://www.abcam.com/cd59-antibody-mem-129-azide-free-ab14221.html

      Read More

      Abcam Scientific Support

      Answered on Jan 15 2013

      Question

      Product code: 9182
      Inquiry: The customer is looking for the secondary anti mouse to stain the cells (ICC/IF) with ab9182. Kindly advise if anti mouse IgG (like ab98794, for instance) can be used or it should be specific anti IgG2a (ab97244). Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on May 02 2012

      Answer

      Thank you for contacting us.
      For ab9182 the ab97244 is indeed a compatible secondary antibody that can be used for ICC/IF.
      The ab98794 is an IgG antibody which can cross react with IgG2a however because we do not have tested data so we can't 100% guarantee this. Our recommendation is to use the compatible secondary antibody.
      Other compatible product is ab97243
      As promotion we are giving away protein ladder free. Please click the below link and recommend this to customer;
      https://www.abcam.com/index.html?pageconfig=resource&rid=14968
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on May 02 2012

      Question

      I purchased ab9182 which is CD59. I have try dilutions from 1/10 to 1/100 and nothing seems to work. I don't get any staining. Today I tried with placenta, 1/20, antigen retrieval with Tris for 32 min and again nothing. I'm using Benchmark automated stainer. Any suggestions??? The lot # is GR36289-3 and order #1021675. Let me know if you need any other information.

      Thanks,

      Read More

      Abcam community

      Verified customer

      Asked on Apr 17 2012

      Answer

      According to our records, ab9182 was proving difficult to use inIHC and we were in contact in order to help resolve the issue.

      Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

      If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

      We wish you the best of luck with your research and look forward to a reply.

      Read More

      Abcam Scientific Support

      Answered on Apr 17 2012

      Question

      No signal in IHC-P on intestine or placental tissue.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 26 2012

      Answer

      Thank you for calling Abcam.

      I am sorry that you were having problems with ab9182 in IHC-P. As we discussed, I am sending you ab9183 as a free of charge replacement and you should have it by 10.30am tomorrow morning. You r new order number is *****.

      If there is anything else I can help you with, please let me know.

      Read More

      Abcam Scientific Support

      Answered on Mar 27 2012

      Question

      I was wondering whether these would crossreact with mouse cd59. Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 03 2003

      Answer

      These two monoclonals (ab9182, ab9183) have not been tested for cross reactivity with mouse CD59.

      Read More

      Abcam Scientific Support

      Answered on Mar 03 2003

      Question

      Regarding my enquiry no. 2381. I was informed by you that my antibody against CD59 (AB9182) should work when SDS was present in the sample buffer. However, as my Western Blots wouldn't work, I ran my own tests using SlotBlot technique. I transferred my samples - known to contain CD59 - to an ECL membrane, which I then probed for CD59 using the antibody that I purchased from you, under varying conditions. I have attached the results in a Word document. The results I have got clearly demonstrates that your antibody does not like sample buffers containing SDS, at least not the one that I have tried (specified in document). Please update your info on this antibody for other customers. It took me some time to reach this conclusion, and I have tried to exchange the antibody a couple of times, so I have almost run out of antibody by now - and of time! Therefore, I would appreciate it if you send me another bottle of this antibody free of charge. If you have any questions or comments, please don't hesitate to contact me! Kindest regards, Karsten Gjessing Jensen

      Read More

      Abcam community

      Verified customer

      Asked on Sep 04 2002

      Answer

      Many thanks for this useful information which we will certainly make available via the online datasheet for this product. I note the originator of this antibody advised that it works under conditions of SDS-PAGE, but the sample buffer should not contain reducing agents (DTT, Mercaptoethanol). WB conditions will vary in different people's hands, and I will certainly include your comments and recommendations on the online datasheet. We will foward a replacement vial to you and thank you again for your feedback.

      Read More

      Abcam Scientific Support

      Answered on Sep 04 2002

      Question

      Will this ab work in Westerns under denaturing conditions, e.g. with SDS?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 27 2002

      Answer

      It works under conditions of SDS-PAGE, but the sample buffer should not contain reducing agents (DTT, Mercaptoethanol). SDS presence in the buffer is fine.

      Read More

      Abcam Scientific Support

      Answered on Aug 27 2002

      1-10 of 11 Abreviews or Q&A

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      For licensing inquiries, please contact partnerships@abcam.com

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