Anti-CD59 抗体 [EPR22394-242] - BSA and Azide free (ab254194)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22394-242] to CD59 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD59 antibody [EPR22394-242] - BSA and Azide free
CD59 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22394-242] to CD59 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cytmore details
適用なし: ICC/IF,IHC-P or WB -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt: Human primary peripheral blood mononuclear cells.
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特記事項
ab254194 is the carrier-free version of ab253239.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22394-242 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab254194の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
関連疾患
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
配列類似性
Contains 1 UPAR/Ly6 domain. -
翻訳後修飾
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
細胞内局在
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
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参照データベース
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
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別名
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
画像
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Flow cytometric analysis of U-937 (human histiocytic lymphoma monocyte, Left panel) / Human primary peripheral blood mononuclear cell (PBMC, Right panel) labeling CD59 with ab253239 at 1/400 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/5000 dilution was used as the secondary antibody.
Negative control: U-937 (PMID:16493049). Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253239).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab254194 は論文での使用が確認できていません。