Anti-CD55 抗体 [MEM-118] (ab1422)

Reviews (1)Q&A (1)References (4)

製品の概要

  • 製品名

    Anti-CD55 antibody [MEM-118]
    CD55 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-118] to CD55

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, Flow Cyt, IP, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Non human primates

  • 免疫原

    Tissue/ cell preparation (Human). (T cell line HPB-ALL).

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 24th Januray 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Proprietary Purification

  • 特記事項（精製）

    Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MEM-118

  • アイソタイプ

    IgM

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 25254972

ターゲット情報

  • 機能

    This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.

  • 組織特異性

    Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.

  • 配列類似性

    Belongs to the receptors of complement activation (RCA) family.
    Contains 4 Sushi (CCP/SCR) domains.

  • ドメイン

    The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.

  • 翻訳後修飾

    The Ser/Thr-rich domain is heavily O-glycosylated.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 55 antibody
      • CD55 antibody
      • CD55 antigen antibody
      • CD55 Cromer blood group system antibody
      • CD55 molecule (Cromer blood group) antibody
      • CD55 molecule antibody
      • CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
      • Cd55a antibody
      • Complement decay accelerating factor antibody
      • Complement decay-accelerating factor antibody
      • Complement decay-accelerating factor, GPI-anchored antibody
      • CR antibody
      • CROM antibody
      • Cromer Blood Group antigen antibody
      • Cromer blood group system antibody
      • DAF antibody
      • Daf-GPI antibody
      • DAF_HUMAN antibody
      • Daf1 antibody
      • Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody
      • Decay accelarating factor 1, isoform CRA_a antibody
      • Decay accelerating factor (GPI-form) antibody
      • Decay Accelerating Factor for Complement antibody
      • Decay accelerating factor GPI-form antibody
      • Decay accelerating factor soluble-form antibody
      • GPI-DAF antibody
      • TC antibody
      see all

    画像

    • Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)
      Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)

      Human peripheral blood lymphocytes stained with ab1422 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab1422, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    参考文献

    ab1422 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1422 は 4 報の論文で使用されています。

    • Cheng L  et al. Complement regulatory proteins in kidneys of patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Clin Exp Immunol 191:116-124 (2018). PubMed: 28940198
    • Kapka-Skrzypczak L  et al. Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells. Oncol Lett 12:1507-1512 (2016). ICC/IF ; Human . PubMed: 27446461
    • Lee MS  et al. Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection. PLoS Pathog 10:e1004412 (2014). ICC/IF ; Human . PubMed: 25254972
    • Cinek T & Horejsi­ V The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol 149:2262-70 (1992). PubMed: 1382093

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD55 antibody [MEM-118]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (intestine)
    Specification
    intestine
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
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    Abcam user community

    Verified customer

    投稿 May 02 2012

    Question

    What is the protocol that was used to test this antibody in IHC-P

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    Answer

    Thank you for contacting Abcam yesterday.

    I asked the supplying lab for the IHC-P protocol they used to verify that the antibody works in IHC but they we do not have the protocol, only information that the suitable working concentration for IHC-paraffin sections by ab1422 is 10 ug/ml.

    If you can send me a copy of the protocol that you are using, including the fix, then I will be happy to see if there are any suggestions that I can make. Also, does the secondary antibody you are using with the ab1422, work well with other primary antibodies and also are you able to stain the same sections successfully with other primary antibodies. I ask as I talked to one of my colleagues and they mentioned that they have seen this problem before and they found that it was due to the fixation process. if the sections are working well with other antibodies then the fixation is most likely not the cause but I wanted to check.

    I look forward to you reply and helping you resolve this issue.

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