Anti-CD55 抗体 [MEM-118] (ab1422)
![Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)](https://www.abcam.co.jp/ps/products/1/ab1422/Images/ab1422-1-ab1422FC.jpg "Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)")
Key features and details
- Mouse monoclonal [MEM-118] to CD55
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
製品の概要
-
製品名
Anti-CD55 antibody [MEM-118]
CD55 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-118] to CD55 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Non human primates
-
免疫原
Tissue/ cell preparation (Human). (T cell line HPB-ALL).
-
特記事項
This product was changed from ascites to tissue culture supernatant on 24th Januray 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 8.0
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
MEM-118 -
アイソタイプ
IgM -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. -
組織特異性
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. -
配列類似性
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains. -
ドメイン
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3. -
翻訳後修飾
The Ser/Thr-rich domain is heavily O-glycosylated. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1604 Human
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
-
別名
- CD 55 antibody
- CD55 antibody
- CD55 antigen antibody
see all
画像
-
Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)
Human peripheral blood lymphocytes stained with ab1422 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab1422, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab1422 は 5 報の論文で使用されています。
- Trimova G et al. Tumour necrosis factor alpha promotes secretion of 14-3-3? by inducing necroptosis in macrophages. Arthritis Res Ther 22:24 (2020). PubMed: 32051018
- Cheng L et al. Complement regulatory proteins in kidneys of patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Clin Exp Immunol 191:116-124 (2018). PubMed: 28940198
- Kapka-Skrzypczak L et al. Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells. Oncol Lett 12:1507-1512 (2016). ICC/IF ; Human . PubMed: 27446461
- Lee MS et al. Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection. PLoS Pathog 10:e1004412 (2014). ICC/IF ; Human . PubMed: 25254972
- Cinek T & Horejsi V The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol 149:2262-70 (1992). PubMed: 1382093