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  1. Link

    cd55-antibody-mem-118-ab1422.pdf

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Immunology Cell Type Markers CD Non-lineage
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Anti-CD55 抗体 [MEM-118] (ab1422)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (5)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)

    Key features and details

    • Mouse monoclonal [MEM-118] to CD55
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgM

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 647 FITC PE

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-CD55 antibody [EPR22362-255] (ab243231)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-CD55 antibody [MEM-118]
      CD55 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [MEM-118] to CD55
    • 由来種

      Mouse
    • アプリケーション

      適用あり: Flow Cytmore details
    • 種交差性

      交差種: Human
      交差が予測される動物種: Non human primates
    • 免疫原

      Tissue, cells or virus corresponding to Human CD55. T cell line HPB-ALL

    • 特記事項

      This product was changed from ascites to tissue culture supernatant on 24th Januray 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 8.0
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Proprietary Purification
    • 特記事項(精製)

      Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
    • ポリ/モノ

      モノクローナル
    • クローン名

      MEM-118
    • アイソタイプ

      IgM
    • 研究分野

      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage

    関連製品

    • Alternative Versions

      • PE Anti-CD55 antibody [MEM-118] (ab130421)
      • Alexa Fluor® 488 Anti-CD55 antibody [MEM-118] (ab187775)
      • Alexa Fluor® 647 Anti-CD55 antibody [MEM-118] (ab239274)
      • FITC Anti-CD55 antibody [MEM-118], prediluted (ab28112)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgM [B11/7] - Isotype control (ab91545)
    • Recombinant Protein

      • Recombinant human CD55 protein (ab168708)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1422の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use 1µg for 106 cells.

    ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

    特記事項
    Flow Cyt
    Use 1µg for 106 cells.

    ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 機能

      This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
    • 組織特異性

      Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
    • 配列類似性

      Belongs to the receptors of complement activation (RCA) family.
      Contains 4 Sushi (CCP/SCR) domains.
    • ドメイン

      The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
    • 翻訳後修飾

      The Ser/Thr-rich domain is heavily O-glycosylated.
    • 細胞内局在

      Cell membrane.
    • Target information above from: UniProt accession P08174 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 1604 Human
      • Omim: 125240 Human
      • SwissProt: P08174 Human
      • Unigene: 126517 Human
      • 別名

        • CD 55 antibody
        • CD55 antibody
        • CD55 antigen antibody
        • CD55 Cromer blood group system antibody
        • CD55 molecule (Cromer blood group) antibody
        • CD55 molecule antibody
        • CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
        • Cd55a antibody
        • Complement decay accelerating factor antibody
        • Complement decay-accelerating factor antibody
        • Complement decay-accelerating factor, GPI-anchored antibody
        • CR antibody
        • CROM antibody
        • Cromer Blood Group antigen antibody
        • Cromer blood group system antibody
        • DAF antibody
        • Daf-GPI antibody
        • DAF_HUMAN antibody
        • Daf1 antibody
        • Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody
        • Decay accelarating factor 1, isoform CRA_a antibody
        • Decay accelerating factor (GPI-form) antibody
        • Decay Accelerating Factor for Complement antibody
        • Decay accelerating factor GPI-form antibody
        • Decay accelerating factor soluble-form antibody
        • GPI-DAF antibody
        • TC antibody
        see all

      画像

      • Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)
        Flow Cytometry - Anti-CD55 antibody [MEM-118] (ab1422)

        Human peripheral blood lymphocytes stained with ab1422 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab1422, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

      プロトコール

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (5)

      ab1422 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab1422 は 5 報の論文で使用されています。

      • Trimova G  et al. Tumour necrosis factor alpha promotes secretion of 14-3-3? by inducing necroptosis in macrophages. Arthritis Res Ther 22:24 (2020). PubMed: 32051018
      • Cheng L  et al. Complement regulatory proteins in kidneys of patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Clin Exp Immunol 191:116-124 (2018). PubMed: 28940198
      • Kapka-Skrzypczak L  et al. Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells. Oncol Lett 12:1507-1512 (2016). ICC/IF ; Human . PubMed: 27446461
      • Lee MS  et al. Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection. PLoS Pathog 10:e1004412 (2014). ICC/IF ; Human . PubMed: 25254972
      • Cinek T & Horejsi­ V The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol 149:2262-70 (1992). PubMed: 1382093

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD55 antibody [MEM-118]

      Good
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (intestine)
      Specification
      intestine
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      None
      Permeabilization
      No
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 May 02 2012

      Question

      What is the protocol that was used to test this antibody in IHC-P

      Read More

      Abcam community

      Verified customer

      Asked on Mar 09 2012

      Answer

      Thank you for contacting Abcam yesterday.

      I asked the supplying lab for the IHC-P protocol they used to verify that the antibody works in IHC but they we do not have the protocol, only information that the suitable working concentration for IHC-paraffin sections by ab1422 is 10 ug/ml.

      If you can send me a copy of the protocol that you are using, including the fix, then I will be happy to see if there are any suggestions that I can make. Also, does the secondary antibody you are using with the ab1422, work well with other primary antibodies and also are you able to stain the same sections successfully with other primary antibodies. I ask as I talked to one of my colleagues and they mentioned that they have seen this problem before and they found that it was due to the fixation process. if the sections are working well with other antibodies then the fixation is most likely not the cause but I wanted to check.

      I look forward to you reply and helping you resolve this issue.

      Read More

      Abcam Scientific Support

      Answered on Mar 09 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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