Anti-CD5 抗体 [EP2952] - BSA and Azide free (ab194821)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2952] to CD5 - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD5 antibody [EP2952] - BSA and Azide free
CD5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2952] to CD5 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WBmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human lymph node lysate IHC-P: Human tonsil tissue. IP: Human lymph node lysate
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特記事項
ab194821 is the carrier-free version of ab75877.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.70 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2952 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 647 Anti-CD5 antibody [EP2952] (ab309505)
- Alexa Fluor® 488 Anti-CD5 antibody [EP2952] (ab311030)
- Alexa Fluor® 594 Anti-CD5 antibody [EP2952] (ab311803)
- Alexa Fluor® 568 Anti-CD5 antibody [EP2952] (ab313086)
- Alexa Fluor® 555 Anti-CD5 antibody [EP2952] (ab313280)
- Anti-CD5 antibody [EP2952] (ab75877)
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab194821の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
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特記事項 |
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IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa. |
ターゲット情報
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機能
May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. -
配列類似性
Contains 3 SRCR domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 921 Human
- Omim: 153340 Human
- SwissProt: P06127 Human
- Unigene: 58685 Human
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別名
- CD 5 antibody
- CD5 antibody
- CD5 antigen (p56 62) antibody
see all
画像
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Anti-CD5 antibody [EP2952] (ab75877) at 1/1000 dilution (Purified) + Human lymph node lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).
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ab75877 showing negative staining in Normal brain tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab75877 showing positive staining in Normal colon lymphocytes tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab75877 showing negative staining in Normal kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab75877 showing negative staining in Skeletal muscle tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab75877 showing positive staining in Normal spleen tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab75877, the same antibody clone in a different buffer formulation.
CD5 was immunoprecipitated from 0.35 mg Human lymph node lysate 10 µg with ab75877 at 1/20 dilution (0.6µg) . VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: Human lymph node lysate 10 µg
Lane 2: abab75877 IP in Human lymph node lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75877 in Human lymph node lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab194821 は論文での使用が確認できていません。