Key features and details
- Rabbit polyclonal to CD47
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
CD47 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to CD47
アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
種交差性交差種: Mouse, Rat, Human
- WB: MCF-7 and SK-OV-3 cell lysates; Mouse lung lysate. IHC: human placenta and human lung cancer tissues. ICC/IF: L929 and U-2 OS cells.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab175388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 35 kDa.|
機能Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
組織特異性Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
配列類似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
Formalin-fixed, paraffin-embedded human placenta tissue stained for CD47 using ab175388 at 1/200 dilution in immunohistochemical analysis.
Immunofluorescence staining of L929 cells stained for CD47 with ab175388 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-CD47 antibody (ab175388) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : SK-OV-3 (human ovary adenocarcinoma cell line) cell lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 90 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for CD47 using ab175388 at 1/200 dilution in immunohistochemical analysis.
Immunofluorescence staining of U-2 OS cells stained for CD47 with ab175388 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
ab175388 は 7 報の論文で使用されています。
- Wang Y et al. Biomimetic Nanotherapies: Red Blood Cell Based Core-Shell Structured Nanocomplexes for Atherosclerosis Management. Adv Sci (Weinh) 6:1900172 (2019). PubMed: 31380165
- Pan Y et al. Single-cell RNA sequencing reveals compartmental remodeling of tumor-infiltrating immune cells induced by anti-CD47 targeting in pancreatic cancer. J Hematol Oncol 12:124 (2019). PubMed: 31771616
- Jellvert Å et al. Castration is a prerequisite for the inhibitory effect of metronomic chemotherapy on the growth of experimental castration-resistant prostate cancer. Acta Oncol 57:895-901 (2018). PubMed: 29299975
- Liu F et al. SRSF10-mediated IL1RAP alternative splicing regulates cervical cancer oncogenesis via mIL1RAP-NF-?B-CD47 axis. Oncogene 37:2394-2409 (2018). PubMed: 29429992
- Wu L et al. Anti-CD47 treatment enhances anti-tumor T-cell immunity and improves immunosuppressive environment in head and neck squamous cell carcinoma. Oncoimmunology 7:e1397248 (2018). PubMed: 29632717
- Bittins M & Wang X TNT-Induced Phagocytosis: Tunneling Nanotubes Mediate the Transfer of Pro-Phagocytic Signals From Apoptotic to Viable Cells. J Cell Physiol 232:2271-2279 (2017). IF . PubMed: 27591547
- Zhang S et al. Acute CD47 Blockade During Ischemic Myocardial Reperfusion Enhances Phagocytosis-Associated Cardiac Repair. JACC Basic Transl Sci 2:386-397 (2017). PubMed: 28920097