遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-CD46 抗体 [EPR4014] - BSA and Azide free (ab271871)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
  • Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4014] to CD46 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Knockout validated
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-CD46 antibody [EPR4014] - BSA and Azide free
    CD46 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4014] to CD46 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Molt-4, Jurkat, HeLa, and K562 cell lysates; Human kidney and Tonsil tissue

  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    ab271871 is the carrier-free version of ab108307. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
特記事項
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.

    • ターゲット情報

    • 機能

      Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.

    • 組織特異性

      Expressed by all cells except erythrocytes.

    • 関連疾患

      Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) [MIM:612922]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations.

    • 配列類似性

      Contains 4 Sushi (CCP/SCR) domains.

    • ドメイン

      Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.

    • 翻訳後修飾

      N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
      Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
      In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by Lck.

    • 細胞内局在

      Cytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
    • Information by UniProt

    • 参照データベース

      • 別名

        • AHUS2 antibody
        • Antigen defined by monoclonal antibody TRA 2 10 antibody
        • Antigen identified by monoclonal antibody TRA 2 10 antibody
        • CD46 antibody
        • CD46 antigen antibody
        • CD46 antigen complement regulatory protein antibody
        • CD46 molecule antibody
        • CD46 molecule complement regulatory protein antibody
        • Complement membrane cofactor protein antibody
        • MCP antibody
        • MCP_HUMAN antibody
        • Measles virus receptor antibody
        • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody
        • Membrane cofactor protein antibody
        • MGC26544 antibody
        • MIC10 antibody
        • TLX antibody
        • TRA2.10 antibody
        • Trophoblast leucocyte common antigen antibody
        • Trophoblast leukocyte common antigen antibody
        • Trophoblast lymphocyte cross reactive antigen antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

        ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded Human tonsil tissue

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

        ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded Human kidney tissue

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

        ab108307 showing positive staining in Normal placenta tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

        ab108307 showing positive staining in Normal uterus tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

        ab108307 showing positive staining in Thyroid gland carcinoma tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

        ab108307 showing positive staining in Colonic adenocarcinoma tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

        ab108307 showing positive staining in Breast carcinoma tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

        ab108307 showing positive staining in Normal breast tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

      • Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)
        Anti-CD46 antibody [EPR4014] - BSA and Azide free (ab271871)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab271871 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab271871 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      There are currently no Customer reviews or Questions for ab271871.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com