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  1. Link

    cd45ro-antibody-uch-l1-ab23.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD45RO 抗体 [UCH-L1] (ab23)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (15)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [UCH-L1] (ab23)
  • Western blot - Anti-CD45RO antibody [UCH-L1] (ab23)

Key features and details

  • Mouse monoclonal [UCH-L1] to CD45RO
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

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関連製品

医薬用外毒物

製品の概要

  • 製品名

    Anti-CD45RO antibody [UCH-L1]
    CD45RO 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [UCH-L1] to CD45RO
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to CD45RO. Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.

  • ポジティブ・コントロール

    • IHC-P: Human tonsil tissue. WB: Human tonsil and thymus tissue lysate.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.2% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    UCH-L1
  • ミエローマ

    P3-NS1/1-Ag4-1
  • アイソタイプ

    IgG2a
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Neuroscience
    • Processes

関連製品

  • Alternative Versions

    • HRP Anti-CD45RO antibody [UCH-L1] (ab187836)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab23の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P (3)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • 関連疾患

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • ドメイン

    The first PTPase domain interacts with SKAP1.
  • 翻訳後修飾

    Heavily N- and O-glycosylated.
  • 細胞内局在

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession P08575 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5788 Human
    • Omim: 151460 Human
    • SwissProt: P08575 Human
    • Unigene: 654514 Human
    • 別名

      • B220 antibody
      • CD45 antibody
      • cd45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • lca antibody
      • Leukocyte common antigen antibody
      • LY 5 antibody
      • LY5 antibody
      • Protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [UCH-L1] (ab23)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [UCH-L1] (ab23)

      ab23 (1 µg/ml) staining CD45RO in human tonsil.
      Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    • Western blot - Anti-CD45RO antibody [UCH-L1] (ab23)
      Western blot - Anti-CD45RO antibody [UCH-L1] (ab23)
      All lanes : Anti-CD45RO antibody [UCH-L1] (ab23) at 5 µg/ml

      Lane 1 : Human tonsil normal tissue lysate - total protein (ab29615)
      Lane 2 : Human thymus tissue lysate - total protein (ab30146)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 131 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?
      Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes


      CD45RO contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    プロトコール

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (15)

    ab23 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab23 は 15 報の論文で使用されています。

    • Yang W  et al. ACE2 Correlated With Immune Infiltration Serves As A Novel Prognostic Biomarker In Clear Cell Renal Cell Carcinoma: Implication For COVID-19. Int J Biol Sci 17:20-31 (2021). PubMed: 33390830
    • Kim HD  et al. Spatial Distribution and Prognostic Implications of Tumor-Infiltrating FoxP3- CD4+ T Cells in Biliary Tract Cancer. Cancer Res Treat 53:162-171 (2021). PubMed: 32878426
    • Huang KC  et al. Prognostic relevance of programmed cell death 1 ligand 2 (PDCD1LG2/PD-L2) in patients with advanced stage colon carcinoma treated with chemotherapy. Sci Rep 10:22330 (2020). PubMed: 33339860
    • Patibandla C  et al. Costus pictus D. Don leaf extract stimulates GLP-1 secretion from GLUTag L-cells and has cytoprotective effects in BRIN-BD11 ß-cells. J Ethnopharmacol 260:112970 (2020). PubMed: 32422353
    • Zhang WH  et al. Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors. J Immunother Cancer 8:N/A (2020). PubMed: 33055204
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45RO antibody [UCH-L1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 12 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45RO antibody [UCH-L1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    Bloxall Endogenous Peroxidase and Alkaline Phosphatase Blocking as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Mr. Joshua Dodge

    Verified customer

    投稿 Jan 22 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45RO antibody [UCH-L1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA
    Permeabilization
    No
    Specification
    Lymph node
    Blocking step
    Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 30 2015

    Question

    Please tell us the necessary dilution for this product ab23 Mouse monoclonal [UCH-L1] to CD45RO lot no GR114857, for IHC-P use.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2013

    Answer


    The concentration for this lot is 1 mg/ml. We haven't specified a dilution range for this product in IHC-P however on the datasheet, IHC-P was successfully performed using 1 ug/ml therefore a 1/1000 dilution. I would suggest starting with this dilution and then optimising it for your particular experiment.

    Read More

    Abcam Scientific Support

    Answered on Mar 20 2013

    Question

    Dear Sir/Madam˜

    I hope you have a great day.


    Does ab23 contain any BSA?

    Our customers do not need BSA containg on these products.


    .

    Read More

    Abcam community

    Verified customer

    Asked on Jul 03 2012

    Answer

    Thank you for contacting us. No, ab23 does not contain any BSA, the antibody is in PBS only.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 03 2012

    Question

    Does the CD45RO (clone UCH-L1) cross-react with cynomolgus?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2001

    Answer

    We have not had any reports of this antibody being used with cynamologous and the nature of the epitope is unknown. A reference to the immunodetection of CD45RO in cynaomolgous as shown below: Li SL et al, Res Virol 1991 Mar-Jun;142(2-3):173-82

    Read More

    Abcam Scientific Support

    Answered on Feb 21 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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