Anti-CD45RA 抗体 [4KB5] - BSA and Azide free (ab256137)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [4KB5] to CD45RA - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD45RA antibody [4KB5] - BSA and Azide free
CD45RA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4KB5] to CD45RA - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human tonsil tissue. Flow Cyt: Human peripheral blood mononuclear cells.
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特記事項
ab256137 is the carrier-free version of ab755.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
4KB5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Affibody® Molecule
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab256137の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
Use a concentration of 0.239 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Flow Cyt |
Use a concentration of 1.017 µg/ml.
|
特記事項 |
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IHC-P
Use a concentration of 0.239 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Use a concentration of 1.017 µg/ml. |
ターゲット情報
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機能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
関連疾患
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
ドメイン
The first PTPase domain interacts with SKAP1. -
翻訳後修飾
Heavily N- and O-glycosylated. -
細胞内局在
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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別名
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD45RA with ab755 at 0.239µg/ml, followed by ready to use secondary. Positive staining on human tonsil tissue is observed. The section was incubated with ab755 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab755).
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD45RA with ab755 at 1.017µg/ml, compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This image was produced using the same antibody clone but in a different formulation ab755, PBS, sodium azide, glycerol and BSA.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab755).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab256137 は 1 報の論文で使用されています。
- Li FJ et al. A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood 105:3295-302 (2005). PubMed: 15626731