Anti-CD45 抗体 [PD7/26 + 2B11] (ab781)

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Key features and details

  • Mouse monoclonal [PD7/26 + 2B11] to CD45
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD45 antibody [PD7/26 + 2B11]
    CD45 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [PD7/26 + 2B11] to CD45

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Fr, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to CD45. Human peripheral blood lymphocytes were used to raise clone PD7/ 26 and isolated neoplastic cells from a T cell lymphoma were used to raise clone 2B11.

  • ポジティブ･コントロール

    • Tonsil.

  • 特記事項

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

アプリケーション

Our Abpromise guarantee covers the use of ab781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

1/40 - 1/100 for 30 - 60 mins at RT (ABC method).

ターゲット情報

  • 機能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

  • 関連疾患

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.

  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.

  • ドメイン

    The first PTPase domain interacts with SKAP1.

  • 翻訳後修飾

    Heavily N- and O-glycosylated.

  • 細胞内局在

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PD7/26 + 2B11] (ab781)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PD7/26 + 2B11] (ab781)

      ab781 - immunohistochemistry

      Formalin fixed parrafin embedded human tonsil stained with CD45 LCA, using ABC and DAB chromagen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PD7/26 + 2B11] (ab781)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PD7/26 + 2B11] (ab781)

      ab781 staining human tonsil by IHC-P.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PD7/26 + 2B11] (ab781)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PD7/26 + 2B11] (ab781)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining CD45 with ab781.

    参考文献

    ab781 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab781 は 5 報の論文で使用されています。

    • Her Z  et al. An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia. J Hematol Oncol 10:162 (2017). IHC-P ; Mouse . PubMed: 28985760
    • Sun JD  et al. Efficacy and safety of the hypoxia-activated prodrug TH-302 in combination with gemcitabine and nab-paclitaxel in human tumor xenograft models of pancreatic cancer. Cancer Biol Ther 16:438-49 (2015). IHC . PubMed: 25679067
    • Kohno H  et al. CCL3 production by microglial cells modulates disease severity in murine models of retinal degeneration. J Immunol 192:3816-27 (2014). Mouse . PubMed: 24639355
    • Han G  et al. Preventive and therapeutic effects of Smad7 on radiation-induced oral mucositis. Nat Med 19:421-8 (2013). Human . PubMed: 23475202
    • DeRose YS  et al. Tumor grafts derived from women with breast cancer authentically reflect tumor pathology, growth, metastasis and disease outcomes. Nat Med 17:1514-20 (2011). Human . PubMed: 22019887

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