Anti-CD45 抗体 [OX30] (ab6329)

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Key features and details

  • Mouse monoclonal [OX30] to CD45
  • Suitable for: Flow Cyt
  • Reacts with: Rat
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-CD45 antibody [OX30]
    CD45 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [OX30] to CD45

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Tissue, cells or virus corresponding to Rat CD45. Rat lymph node cells and glycoproteins.

  • ポジティブ･コントロール

    • Flow Cyt: Lewis rat splenocytes.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

アプリケーション

Our Abpromise guarantee covers the use of ab6329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 0.2 µg/ml.

ターゲット情報

  • 機能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

  • 関連疾患

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.

  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.

  • ドメイン

    The first PTPase domain interacts with SKAP1.

  • 翻訳後修飾

    Heavily N- and O-glycosylated.

  • 細胞内局在

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    画像

    • Flow Cytometry - Anti-CD45 antibody [OX30] (ab6329)
      Flow Cytometry - Anti-CD45 antibody [OX30] (ab6329)

      Lewis rat splenocytes stained with ab6329 (right) or mouse IgG2aκ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6329) or mouse IgG2aκ Isotype (ab18450) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.

      The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

      Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

       

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab6329 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6329 は 1 報の論文で使用されています。

    • Woollett GR  et al. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes. Eur J Immunol 15:168-73 (1985). PubMed: 2578966

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    1-5 of 5 Abreviews or Q&A

    Question

    Our customer wants to know the positive control of ab6329. She will perform IHC. Could you recommend the positive control?

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    Answer

    Thank you for your enquiry. Whilst we do not have a recommended positive control the following publication employed leukocytes as a control for the detection of CD45 transcripts in rat cells. De Dios I, Ramudo L, Alonso JR, Recio JS, Garcia-Montero AC, Manso MA. CD45 expression on rat acinar cells: involvement in pro-inflammatory cytokine production. FEBS Lett. 2005 Nov 21;579(28):6355-60. Epub 2005 Oct 24. PMID: 16263122 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    Please comment on the suitability of these five antibodies for use in IHC-P on rat tissue.

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    Answer

    You recently spoke with my colleague Maud Osborne regarding the use of a number of anti CD antibodies in IHC on paraffin embedded rat sections. As you have seen, none of the antibodies had been tested in the exact conditions you wish to try although my comments about their use are below. ab26239 (CD44) - has been tested in IHC-P on mouse & human although not yet tested in rat. As it has been shown to work in mouse I think it's a good bet that it will work in rat although there's no guarantee. ab6329 (CD45) - has been tested in IHC on Frozen sections but not on paraffin sections and shown to cross react with rat. ab22479 (CD45) - raised in rat and shown to cross react with rat - take care when using this antibody in IHC to block any endogenous rat IgGs that may bind to your anti rat secondary ab23844 (CD44) - works in IHC-P and cross reacts with cow, dog, goat, horse, pig, sheep so again although there's no guarantee, I think it's reasonable to think that this will cross react with rat too! ab19673 (CD105) - cross reacts with human and works in IHC-P I hope this is a helpful summary.

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    Question

    Has this antibody been tested in IHC-paraffin or IHC-frozen, or both?

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    Answer

    Thank you for your patience. This product has been tested in IHC-frozen, used at dilutions between 1:25 - 1:200. I have updated the datasheet to reflect this information for future use. To the best of my knowledge, it has not been tested in IHC-paraffin. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    Is this a mouse or rat monoclonal? In the raised in section is says rat, but the description is mouse monoclonal. If rat, has it been tested in formalin fixed paraffin embedded sections?

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    Answer

    Unfortunately, MAS 703 has not been tested on formalin fixed tissue. The odds are that it will work, but we can't say for certain until tested.

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    Question

    Do you have any info regarding cross reactivity with mice tissue? Does it work on formalin fixed, paraffin embedded tissue? How long do you incubate in this primary? Does this require antigen retrieval? If so, what is the method/buffer/enzyme you use? Thank-you

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    Answer

    This antibody is raised in mouse and so is difficult to use on mouse tissue without getting problems with background staining. We have no records of this antibody working on paraffin sections.

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