Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH 6)
Permeabilization
No
Specification
Uterus
Blocking step
Background Sniper, from Biocare Medical (ref nr BS966L) as blocking agent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: (0,02 M) TBS +0,05% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: MACH 3 Mouse AP-Polymer Detection from Biocare Med
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 20 2021