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    cd45-antibody-ab10558.pdf

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Anti-CD45 抗体 (ab10558)

  • Datasheet
  • SDS
Reviews (50)Q&A (30)References (198)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)
  • Flow Cytometry - Anti-CD45 antibody (ab10558)
  • Western blot - Anti-CD45 antibody (ab10558)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)
  • Western blot - Anti-CD45 antibody (ab10558)
  • Flow Cytometry - Anti-CD45 antibody (ab10558)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)

Key features and details

  • Rabbit polyclonal to CD45
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

こちらの製品もご検討ください

Biochemical
RWJ-60475 (AM)3, CD45 tyrosine phosphatase inhibitor (ab141729)
タンパク質
Recombinant human CD45 protein (Fc Chimera Active) (ab214825)

関連製品

このターゲットにつきましては、リコンビナント・ウサギ・モノクローナル抗体も販売しています

製品の概要

  • 製品名

    Anti-CD45 antibody
    CD45 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to CD45
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: Flow Cyt, WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Human CD45 aa 900-1000 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab17553)

  • 特記事項

      

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Signal Transduction
    • Antibodies
    • cd45

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CD45 protein (Fc Chimera Active) (ab214825)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Alexa Fluor® 488 Anti-CD45 antibody [F10-89-4] (ab197730)
    • Anti-CD31 antibody [P2B1] (ab24590)
    • Anti-CD45 antibody [F10-89-4] - Hematopoietic Stem Cell Marker (ab30470)
    • Anti-CD68 antibody [FA-11] (ab53444)

アプリケーション

Our Abpromise guarantee covers the use of ab10558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/500. Detects a band of approximately 190 kDa (predicted molecular weight: 147 kDa).
IHC-P Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 関連疾患

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • ドメイン

    The first PTPase domain interacts with SKAP1.
  • 翻訳後修飾

    Heavily N- and O-glycosylated.
  • 細胞内局在

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession P08575 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5788 Human
    • Entrez Gene: 19264 Mouse
    • Entrez Gene: 24699 Rat
    • Omim: 151460 Human
    • SwissProt: P08575 Human
    • SwissProt: P06800 Mouse
    • SwissProt: P04157 Rat
    • Unigene: 654514 Human
    • Unigene: 391573 Mouse
    • Unigene: 90166 Rat
    see all
  • 別名

    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • Protein tyrosine phosphatase, receptor type C antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)

    ab10558 (1:40) staining CD45 in paraffin-embedded human tonsil (left panel) using an automated system (Ventana Discovery). Right-hand panel shows negative control (no primary antibody).
    Using this protocol there is strong membrane staining of B cells in the germinal centres and mantle zone of the follicles and scattered cells of the interfollicular areas (paracortical T and B cells). There is a mild to moderate degree of cytoplasmic staining associated with the membrane staining in these specific cells.

    Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Extended Retrieval programme. Slides were blocked in 3% H2O2 /4 min/ 37°C and incubated with ab10558 (1:40 dilution / 1 hour/ 37°C). Sections then blocked (4mins/ 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min/ 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC (HRP-DAB) system (16 min/ 37°C) before being counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)Lorenzi T et al., PLos One 7:e35232 (2012), Fig 4, doi: 10.1371/journal.pone.0035232 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ab10558 staining CD45 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Negative control is shown in panel. Blocking was with horse serum (1/75) for 1 hour at room temperature. Samples were incubated with primary antibody (1/10) overnight at 4°C. A Biotin-conjugated Horse anti-mouse polyclonal (1/200) was used as the secondary antibody.

  • Flow Cytometry - Anti-CD45 antibody (ab10558)
    Flow Cytometry - Anti-CD45 antibody (ab10558)

    Overlay histogram showing Jurkat cells stained with ab10558 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10558, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/1000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Western blot - Anti-CD45 antibody (ab10558)
    Western blot - Anti-CD45 antibody (ab10558)
    All lanes : Anti-CD45 antibody (ab10558) at 1/500 dilution

    Lane 1 : Jurkat Whole Cell Lysate
    Lane 2 : Jurkat Whole Cell Lysate with Human CD45 peptide (ab17553)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Predicted band size: 147 kDa


    Exposure time: 3 minutes


    Secondary antibody - goat anti-rabbit H&L (HRP) (ab6721)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)

    IHC image of CD45 antibody staining in a section of formalin-fixed paraffin-embedded normal human spleen* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab10558, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-CD45 antibody (ab10558)
    Western blot - Anti-CD45 antibody (ab10558)
    All lanes : Anti-CD45 antibody (ab10558) at 1 µg/ml

    Lane 1 : Jurkat (Human) Whole Cell Lysate
    Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 3 : Spleen (Mouse) Tissue Lysate
    Lane 4 : Spleen (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 147 kDa
    Observed band size: 190 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 230 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab10558 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    CD45 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

  • Flow Cytometry - Anti-CD45 antibody (ab10558)
    Flow Cytometry - Anti-CD45 antibody (ab10558)This image is courtesy of an abreview submitted by Kirk Mcmanu.

    Asynchronous KM-H2 cells were pelleted and labeled by indirect immunofluorescence. Cells were stained with ab10558 (1/200) for 30min at 4'C, washed and then stained with goat anti-rabbit alexafluor 488 (1/200). Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive Based on the accompanying image, approximately 8.4% of cells exhibited positive staining for anti-CD45. Since KM-H2 are known to have low levels of CD45 transcripts they are expected to have low levels of CD45, which is reflected in the ~8%. This image is from an Abreview.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (ab10558)This image is courtesy of an anonymous abreview.

    Immunohistochemical analysis of formaldehyde fixed human cephalic sections. Primary antibody ab10558 to CD45 incubated at a concentration of 1/100 for 4°C for 18 hours. Secondary antibody used was a goat anti-rabbit congugated to biotin at a 1/200 dilution. Blocking was done with serum at a 10% concentration for 1 hour at 25°C.

    See Abreview

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (198)

    ab10558 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab10558 は 198 報の論文で使用されています。

    • Xie S  et al. Andrographolide Protects Against Adverse Cardiac Remodeling After Myocardial Infarction through Enhancing Nrf2 Signaling Pathway. Int J Biol Sci 16:12-26 (2020). PubMed: 31892842
    • Álvarez-Cilleros D  et al. Cocoa diet modulates gut microbiota composition and improves intestinal health in Zucker diabetic rats. Food Res Int 132:109058 (2020). PubMed: 32331673
    • Fan Z  et al. MicroRNA-24 attenuates diabetic vascular remodeling by suppressing the NLRP3/caspase-1/IL-1ß signaling pathway. Int J Mol Med 45:1534-1542 (2020). PubMed: 32323758
    • Li R  et al. Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization. Arthritis Res Ther 22:75 (2020). PubMed: 32272965
    • Park DY  et al. Engineered cartilage utilizing fetal cartilage-derived progenitor cells for cartilage repair. Sci Rep 10:5722 (2020). PubMed: 32235934
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 80 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (palatine tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    palatine tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 05 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 05 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Ph:6.0
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 08 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 01 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD45 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (liver)
    Permeabilization
    No
    Specification
    liver
    Blocking step
    0.4%Tween in PB bufer+10%NGS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 01 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Cornea)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate
    Permeabilization
    Yes - tween20
    Specification
    Cornea
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 25 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TrisEDTA pH9
    Permeabilization
    Yes - 0.1% Tween in PBS
    Specification
    Lymph node
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 02 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD45 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MC3T3)
    Permeabilization
    Yes - NP40
    Specification
    MC3T3
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
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    Abcam user community

    Verified customer

    投稿 Oct 16 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD45 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
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    Abcam user community

    Verified customer

    投稿 Aug 27 2019

    Western blot abreview for Anti-CD45 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    Abcam user community

    Verified customer

    投稿 Aug 16 2019

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