Anti-CD44 抗体 [F10-44-2] (ab6124)

Reviews (5)Q&A (7)References (53)
Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (ab6124)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)

Key features and details

  • Mouse monoclonal [F10-44-2] to CD44
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-CD44 antibody [F10-44-2]
    CD44 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [F10-44-2] to CD44

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt, ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to CD44.

  • ポジティブ･コントロール

    • In Flow Cytometry, this antibody gave a positive signal in peripheral blood lymphocytes. In IHC, this antibody gave a positive signal in human kidney carcinoma sections. ICC/IF: A431 cell line.

  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6124の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P (3)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt
Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

  • 組織特異性

    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

  • 配列類似性

    Contains 1 Link domain.

  • ドメイン

    The lectin-like LINK domain is responsible for hyaluronan binding.

  • 翻訳後修飾

    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • CDW44 antigen antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • HUTCHI antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • INLU-related p80 Glycoprotein antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH I antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      • Soluble CD44 antibody
      see all

    画像

    • Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124)
      Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124)

      Overlay histogram showing peripheral blood lymphocytes stained with ab6124 (red line). The cells were incubated with the antibody (ab6124, 0.5µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)Image from Le Bras GF et al., PLoS One. 2011;6(11):e27063. Epub 2011 Nov 1. Fig 3.; doi:10.1371/journal.pone.0027063; November 1, 2011, PLoS ONE 6(11): e27063.
      Immunohistochemical analysis of Human esophogeal keratinocytes, staining CD44 with ab6124.

      Keratinocytes were cultured in vivo to form an epithelium for 15 days before fixing in formaldehyde and embedding in paraffin. Primary antibody was incubated overnight at 4°C and secondary antibody for 30 minutes at 37°C. Staining was detected using DAB.
    • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (ab6124)
      Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (ab6124)

      ab6124 staining CD44 in A431 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6142 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)
      IHC image of CD44 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6124, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    参考文献 (53)

    ab6124 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6124 は 53 報の論文で使用されています。

    • Yadav VK  et al. Preclinical Evaluation of the Novel Small-Molecule MSI-N1014 for Treating Drug-Resistant Colon Cancer via the LGR5/ß-catenin/miR-142-3p Network and Reducing Cancer-Associated Fibroblast Transformation. Cancers (Basel) 12:N/A (2020). PubMed: 32560222
    • Farfán N  et al. SNAIL expression correlates with the translocation of syndecan-1 intracellular domain into the nucleus in prostate cancer cell lines. Int J Mol Med 45:1073-1080 (2020). PubMed: 32124938
    • Elkashty OA  et al. Cancer stem cells enrichment with surface markers CD271 and CD44 in human head and neck squamous cell carcinomas. Carcinogenesis 41:458-466 (2020). PubMed: 31742606
    • Liang Z  et al. Aromatase-induced endogenous estrogen promotes tumour metastasis through estrogen receptor-a/matrix metalloproteinase 12 axis activation in castration-resistant prostate cancer. Cancer Lett 467:72-84 (2019). PubMed: 31499120
    • Chen C  et al. FOXO1 associated with sensitivity to chemotherapy drugs and glial-mesenchymal transition in glioma. J Cell Biochem 120:882-893 (2019). PubMed: 30216501
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD44 antibody [F10-44-2]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Ahmar Aziz

    Verified customer

    投稿 Jul 08 2016

    Western blot abreview for Anti-CD44 antibody [F10-44-2]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (BEL 7404)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    40 µg
    Treatment
    80 µM Doxorubicin for 8hrs/24hrs
    Specification
    BEL 7404
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. guoqing zhu

    Verified customer

    投稿 Jan 22 2016

    Question

    Customer inquiry regarding 5 antibodies in the Human Mesenchymal Stromal Cell Marker panel:
    CD44 (ab6124)
    CD45 (ab10559)
    CD90 (ab23894)
    CD105 (ab44967)
    CD29 (ab52971)

    Customer wondering which antibodies are positive mesenchymal stromal cell markers and which are negative. Also wondering what flow cytometry procedure is in terms of gating and the expected color associated with each antibody.

    Read More
    Answer

    Thank you for contacting Abcam.
    Earlier today we discussed the Human Mesenchymal Stromal Cell Marker panel ab93758. Based on the information on the datasheet, I compiled the following information for you:
    1. Positive selection (CD105, CD29, CD44, CD90)
    2. Negative selection (CD45)
    The stem cell panel ab93758 consists of 5 unconjugated primary antibodies. These antibodies can be used with the secondary antibodies against mouse or rabbit of your choice. You can therefore adapt the fluorochrome choice to the ability of your machine.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    I would like to know whether ab6124 is able to react against CD44s and CD44v molecule on liver cancer cells.

    Read More
    Answer

    Thank you for contacting us.

    Based on the information from the journal Jpn J. Cancer Res 89 283-90 March 1989 (Yutaka Tokue et. al.), it states that this monoclonal antibody can recognises CD44s. Although the experiment sample used in this study was breast tumour cells but I believe the molecule should be the same in liver cells.

    I hope this information is helpful to you. Please do not hesitate to contact us should you need any further information.

    Read More

    Question

    How was your antiCD44 purified, was this done by ion exchange or using a different method? Seeing how the product datasheet is a bit unclear about this.

    Waiting for your reply and thank you for your kind cooperation.

    Read More
    Answer

    Thank you for contacting Abcam regarding ab6124.


    This ab was affinity purified on a protein G column. Please let me know if this information is sufficient or if you require additional details.


    I hope this information is helpful. Do not hesitate to contact me if you have any additional questions or concerns.

    Read More

    Question

    Hi,
    a customer of ours is interested in your ab93758. She has co-coltures of stromal mesenchymal stem cellsand T lymphocytes isolated from bone.
    She would like to detect only stromal mesenchymal stem, but not T lymphocytes. Can you please let me know if among the markers (CD44, CD45, CD90 CD29 and CD105) included within ab93758, there is some marker able to detect also T lymphocytes?

    Thanks in advance and kind regards

    Read More
    Answer

    Thank you for your enquiry and interest in our products.

    I can confirm that this panel contains 5 primary antibodies which recognize human mesenchymal stromal cells: ab6124, ab10559, ab23894, ab44967, ab52971. The datasheets can be found at these sites:

    https://www.abcam.com/cd44-antibody-f10-44-2-ab6124.html

    https://www.abcam.com/cd45-antibody-ab10559.html

    https://www.abcam.com/cd90--thy1-antibody-af-9-ab23894.html

    https://www.abcam.com/cd105-antibody-105c02-ab44967.html

    https://www.abcam.com/integrin-beta-1-antibody-ep1041y-carboxyterminal-end-ab52971.html

    There are some very informative and useful websites summarizing the different CD markers. You may wish to recommend to your customer for further consideration:

    https://docs.abcam.com/pdf/immunology/cdantigen_poster.pdf

    http://pathologyoutlines.com/cdmarkers.html

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Product code: 93574
    Inquiry: Our customer is looking for the following : CD44 with the clone H90" . The item we offered against his request is AB93574 which include Clone F10-44-2 not clone 'H90'. Kindly advice which Item from ABCAM matches his request. Thank you

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately, we do not have an anti-CD44 antibody from the H90 clone in our catalogue. We do however have several other antibodies against this target which may be suitable for your customer. The catalogue number you mentioned (ab93574) does not match to an anti-CD44 antibody. The clone mouse monoclonal anti-CD44 clone F10-44-2 is available with many different conjugates which can be viewed from the following link:

    https://www.abcam.com/index.html?pageconfig=searchresults&search=cd44&pt=1&sk=clonenum&sv=F10-44-2&sn=F10-44-2&l=3&fViewMore=1

    The suitability of this clone will depend on the application and samples your customer intends to use. If you could give me a few more details about this I may be able to advise further as to whether this clone would be suitable or if one of our other anti-CD44 antibodies would be more appropriate.

    I hope this information has been of some help. If you would like any further assistance please do let me know.

    Read More

    Question

    Here you are my answers to abcam in details regarding my project so please forward to them and hopfuly I can get their guide asap. I am isolating human hertwig's epithelial root sheath (HERS) cells from periodontal ligament (PDL). So the cells that I am isolating are epithelial that should express these two epithelial markers as published in the literature (E-cadherin and Pan-cytokeratin) and will compare them with Stem cells from Human exfoliated deciduous teeth (SHED) using another two anibodies (CD44) that should be highly expressed in SHED than HERS and (CD73) that will be expressed similarly in both cells. So for characterization I ordered these antibodies from abcam: 1-      Mouse monoclonal (HECD-1) to E Cadherin (ab1416) 2-      Mouse monoclonal (PCK-26) to pan Cytokeratin (ab6401) 3-      Mouse monoclonal (F10-44-2) to CD44 (ab6124) 4-      Mouse monoclonal (7G2) to CD73 (ab54217) -          Immunofluorescence staining steps (first time) HERS were cultured in 4 well culture slide and the second day we did the following: a-      Discard the medium b-     Wash 3 times with PBS each time two mins c-      Add 500 µL methanol to each well for 20 mins at 4°C d-     Discard methanol and wash 3 times with PBS each time two mins e-      Add 7 drops of blocking reagent (from Millipore/ comes in the kit of immunoperoxidase secondary detection system) keep it at room temperature for 1 hr. f-       Discard blocking reagent and add the primary antibodies (1:50 ratio) (each antibody was applied separately in each well of the same chamber slide) (we diluted the antibodies using PBS) g-      Keep the chamber slide in 4°C for overnight. h-     Then discard the antibodies, wash 3 times with PBS each time two mins i-        Add 500 µL of secondary antibody (anti mouse FITC) to each well and keep it for 1 hr at room temperature j-       Discard secondary antibody and wash 3 times with PBS each time two mins k-     Add 500 µL of DAPI to each well keep it for 1 hr in a dark room l-        Discard DAPI and wash properly with PBS m-   The cover of the chamber slide was chopped and fluorescence mounting medium was applied (from Dako: S302380) n-     Cover slip was placed and the observed under fluorescence microscope image analyzer Under microscope: We observed the following (as I sent you previously some images); Green cells with green background and blues nucleus. So how to get black background with only green cells and blue nucleus as we got used for immunofluoescence images? -          We did optimize the method (second time) using the same previous procedures but with primary antibodies 1:300 but we got the same result. -          We did optimize the method (third time) using the same previous steps but with these changes: a-      We used PBS with Tween-20 for proper washing b-     Apply methanol and keep it in -20°C for 5 mins c-      Primary antibodies were applied with ratio (1:500) and keep it for 9 hrs at 4°C d-     DAPI was applied for half hr in a dark room. -          But under microscope we got the same result. So we are planning to use primary antibodies 1:1000 and keep it for 3 hrs but we do really need your recommendation and help to get the correct result before we run the test. And waiting for your new quotation please as I did email you. Best regards,    

    Read More
    Answer

    Thank you for contacting us. The background should be black. I am sorry we never had a question like this before. It looks like this problem might be due to instrumentation then antibodies itself. I have following recommendations that might help; - Use less number of cells per well. - Check the viability and passage level of cells. - The cells must be healthy with low number of passages. - Avoid drying out plate between washings. - Check microscope settings. - Check if the blocking is sufficiently done. You can also use 3-5% BSA for 1-2 hour in PBST for blocking. - Use more diluted primary antibodies. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody [F10-44-2]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (urinary bladder)
    Specification
    urinary bladder
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    Yes - Triton X100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Read More

    Ms. whan kim

    Verified customer

    投稿 May 18 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody [F10-44-2]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin tumor)
    Specification
    Skin tumor
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 27 2009

    1-10 of 12 Abreviews or Q&A

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