Anti-CD44 抗体 [DF1485] (ab270255)
![Immunofluorescence - Anti-CD44 antibody [DF1485] (ab270255)](https://www.abcam.co.jp/ps/products/270/ab270255/Images/ab270255-381082-anti-cd44-antibody-df1485-if-hela-cells.JPG "Immunofluorescence - Anti-CD44 antibody [DF1485] (ab270255)")
Key features and details
- Mouse monoclonal [DF1485] to CD44
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD44 antibody [DF1485]
CD44 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [DF1485] to CD44 -
由来種
Mouse -
特異性
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin.
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Tested Applications & Species
See all applications and species dataApplication Species IF HumanIHC-P Human
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免疫原
Full length native protein (purified) corresponding to Human CD44. Purified CD44 antigen (PGp-1) from lymphocyte membrane
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ポジティブ･コントロール
- IHC-P: Human breast tissue. ICC/IF:HeLa cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A/G purified -
特記事項（精製）
Purified from Bioreactor Concentrate -
ポリ/モノ
モノクローナル -
クローン名
DF1485 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IF |
Human
|
| IHC-P |
Human
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| All applications |
Mouse
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IF |
Use a concentration of 1 - 2 µg/ml.
|
|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
IF
Use a concentration of 1 - 2 µg/ml. |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
組織特異性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
配列類似性
Contains 1 Link domain. -
ドメイン
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻訳後修飾
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
- Omim: 107269 Human
- SwissProt: P16070 Human
- SwissProt: P15379 Mouse
- Unigene: 502328 Human
- Unigene: 423621 Mouse
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別名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
画像
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Immunofluorescence - Anti-CD44 antibody [DF1485] (ab270255)
Confocal immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD44 using ab270255 at 2 μg/ml followed by a CF488 secondary antibody (Green). Nuclei are stained using Reddot (Red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [DF1485] (ab270255)](https://www.abcam.co.jp/ps/products/270/ab270255/Images/ab270255-381081-anti-cd44-antibody-df1485-ihc-human-breast-tissue.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [DF1485] (ab270255)Formalin-fixed, paraffin-embedded human breast tissue stained for CD44 using ab270255 at 2 μg/ml in imunnohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab270255 は論文での使用が確認できていません。
