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Anti-CD44 抗体 [C44Mab-5] - BSA and Azide free (ab264546)

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Western blot - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
  • Flow Cytometry - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
  • Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [C44Mab-5] to CD44 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-CD44 antibody [C44Mab-5] - BSA and Azide free
    CD44 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [C44Mab-5] to CD44 - BSA and Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • ポジティブ･コントロール

    • WB: MDA-MB-231 whole cell lysate. IHC-P: Human lung carcinoma and skin tissue. Flow: MDA-MB-231 cells

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264546 is the carrier-free version of ab264539. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab264546の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 82 kDa (predicted molecular weight: 81 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 82 kDa (predicted molecular weight: 81 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

  • 組織特異性

    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

  • 配列類似性

    Contains 1 Link domain.

  • ドメイン

    The lectin-like LINK domain is responsible for hyaluronan binding.

  • 翻訳後修飾

    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • CDW44 antigen antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • HUTCHI antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • INLU-related p80 Glycoprotein antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH I antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      • Soluble CD44 antibody
      see all

    画像

    • Western blot - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
      Western blot - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
      All lanes : Anti-CD44 antibody [C44Mab-5] (ab264539) at 1.226 µg/ml

      Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 81 kDa
      Observed band size: 82 kDa why is the actual band size different from the predicted?


      Exposure time: 70 seconds


      Blocking/diluting buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264539).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)

      Formalin-fixed, paraffin-embedded Human lung carcinoma tissue stained for CD44 using ab264539 at 0.253 μg/mL followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) in immunohistochemical analysis. Counterstained with Hematoxylin. Membranous staining on Human lung carcinoma. The section was incubated with ab264539 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

      Secondary antibody only control: Used PBS instead of the primary antibody, secondary antibody was a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264539).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)

      Formalin-fixed, paraffin-embedded Human skin tissue stained for CD44 using ab264539 at 0.253 μg/mL followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) in immunohistochemical analysis. Counterstained with Hematoxylin. Membranous staining on human skin. The section was incubated with ab264539 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

      Secondary antibody only control: Used PBS instead of the primary antibody, secondary antibody was a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264539).

    • Flow Cytometry - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
      Flow Cytometry - Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)

      Flow cytometric analysis of MDA-MB-231 (Human breast adenocarcinoma epithelial cell) cell line labeling CD44 (Red) using ab264539 at 1.055 μg/mL followed by Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution. Mouse monoclonal IgG was used as the isotype control (Black). Cell without incubation with primary antibody and secondary antibody (Blue). Gated ov viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264539).

    • Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)
      Anti-CD44 antibody [C44Mab-5] - BSA and Azide free (ab264546)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab264546 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab264546 は論文での使用が確認できていません。

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