Anti-CD44 抗体 [156-3C11] (ab16728)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)](https://www.abcam.co.jp/ps/products/16/ab16728/Images/ab16728-25573-anti-cd44-antibody-156-3c11-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)")
Key features and details
- Mouse monoclonal [156-3C11] to CD44
- Suitable for: ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human, Baboon
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-CD44 antibody [156-3C11]
CD44 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [156-3C11] to CD44 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-P, Flow Cytmore details -
種交差性
交差種: Human, Baboon -
免疫原
Tissue, cells or virus corresponding to Human CD44. Stimulated human leukocytes
-
ポジティブ･コントロール
- Tonsil, HeLa cell line
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
156-3C11 -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16728の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100.
|
|
| IHC-P | (2) |
1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Flow Cyt |
1/1000.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
| 特記事項 |
|---|
|
ICC/IF
1/100. |
|
IHC-P
1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Flow Cyt
1/1000. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
ターゲット情報
-
機能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
組織特異性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
配列類似性
Contains 1 Link domain. -
ドメイン
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻訳後修飾
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 101008690 Baboon
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
-
別名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)This image is courtesy of an anonymous Abreviewab16728 at 1/50 dilution staining baboon skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were fixed with formaldehyde and blocked with a 1% Hydrogen peroxide / Methanol solution prior to incubation with the antibody for 1 hour. A biotinylated swine anti-goat, mouse, rabbit antibody was used as the secondary.
-
![Flow Cytometry - Anti-CD44 antibody [156-3C11] (ab16728)](https://www.abcam.co.jp/ps/products/16/ab16728/Images/ab16728-199442-anti-cd44-antibody-156-3c11-flow-cytometry.jpg)
Flow Cytometry - Anti-CD44 antibody [156-3C11] (ab16728)Human peripheral blood lymphocytes stained with ab16728 (red). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab16728, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)](https://www.abcam.co.jp/ps/products/16/ab16728/Images/ab16728-25572-anti-cd44-antibody-156-3c11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)ab16728 staining human tonsil by IHC-P. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [156-3C11] (ab16728)](https://www.abcam.co.jp/ps/products/16/ab16728/Images/ab16728-25570-anti-cd44-antibody-156-3c11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [156-3C11] (ab16728)ICC/IF image of ab16728 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16728, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab16728 は 5 報の論文で使用されています。
- Sanada TJ et al. Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study. PLoS One 14:e0214654 (2019). PubMed: 30925179
- Virga J et al. Extracellular matrix differences in glioblastoma patients with different prognoses. Oncol Lett 17:797-806 (2019). PubMed: 30655832
- Manonelles A et al. The Presence of Urinary Renal Progenitor Cells in Stable Kidney Transplant Recipients Anticipates Allograft Deterioration. Front Physiol 9:1412 (2018). PubMed: 30364198
- Fatima H et al. Parietal epithelial cell activation marker in early recurrence of FSGS in the transplant. Clin J Am Soc Nephrol 7:1852-8 (2012). IHC ; Human . PubMed: 22917699
- Smeets B et al. Parietal epithelial cells participate in the formation of sclerotic lesions in focal segmental glomerulosclerosis. J Am Soc Nephrol 22:1262-74 (2011). IHC ; Human . PubMed: 21719782