Anti-CD43 抗体 [SPN/1766R] (ab218523)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SPN/1766R] to CD43
- Suitable for: WB, ICC, Flow Cyt, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-CD43 antibody [SPN/1766R]
CD43 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SPN/1766R] to CD43 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human CD43 aa 1-400.
Database link: P16150 -
ポジティブ・コントロール
- IHC-P: Human tonsil tissue; WB: K562 cell lysate; ICC and Flow Cyt: K562 cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
SPN/1766R -
アイソタイプ
IgG -
軽鎖の種類
kappa -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab218523の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa.
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ICC |
Use a concentration of 1 - 2 µg/ml.
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Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. |
ICC
Use a concentration of 1 - 2 µg/ml. |
Flow Cyt
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
組織特異性
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
翻訳後修飾
Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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別名
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
画像
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Immunocytochemistry analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 with ab218523 at 2 µg/mL followed by Goat anti-Rabbit- IgG-CF488 (green). The nuclear counterstain is NucSpot (red).
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Flow Cytometry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 with ab218523 at 2 µg/106 cells followed by Goat anti-Rabbit- IgG-CF488 (Blue); Isotype Control (Red).
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Anti-CD43 antibody [SPN/1766R] (ab218523) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Predicted band size: 40 kDaCD43 protein has multiple sites of glycosylation which increases the apparent molecular weight in Western blot.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling CD43 with ab218523 at 1 μg/ml.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab218523 は論文での使用が確認できていません。