Anti-CD42b 抗体 [SP219] (ab183345)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP219] to CD42b
- Suitable for: Flow Cyt (Intra), IHC-Fr, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD42b antibody [SP219]
CD42b 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP219] to CD42b -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-Fr, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Dog -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human spleen and bone marrow and mouse spleen tissues. IHC-Fr: Mouse spleen tissue Flow Cyt (intra): Mouse splenocytes cells, Jurkat and HEL cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP219 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183345の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-Fr | (1) |
1/100.
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IHC-P | (5) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
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Flow Cyt (Intra)
1/20 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-Fr
1/100. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. -
関連疾患
Non-arteritic anterior ischemic optic neuropathy
Bernard-Soulier syndrome
Bernard-Soulier syndrome A2, autosomal dominant
Pseudo-von Willebrand disease -
配列類似性
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
翻訳後修飾
Glycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 403638 Dog
- Entrez Gene: 2811 Human
- Entrez Gene: 14723 Mouse
- Omim: 606672 Human
- SwissProt: Q28256 Dog
- SwissProt: P07359 Human
- SwissProt: O35930 Mouse
- Unigene: 1472 Human
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別名
- Antigen CD42b alpha antibody
- Antigen CD42b-alpha antibody
- BDPLT1 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded (A) Bone marrow tissue from wild-type C57BL/6JGpt mice (B) Bone marrow tissue from CD42b knockout mice staining with ab183345 at 1/500 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Bone marrow tissue from wild-type C57BL/6JGpt mice and no staining on (B) Bone marrow tissue from CD42b knockout mice. The section was incubated with ab183345 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD42b-KO homozygous mice (Strain ID: T028631).
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Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice, (B) Spleen tissue from CD42b knockout mice staining with ab183345 at 1/500 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from CD42b knockout mice. The section was incubated with ab183345 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD42b-KO homozygous mice (Strain ID: T028631).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD42b with ab183345 at 1/100 dilution (1.94 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on platelets and megakaryocytes in the mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183345 for 10 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD42b with ab183345 at 1/100 dilution (1.94 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on platelets in the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183345 for 10 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bone marrow tissue sections labeling CD42b with ab183345 at 1/100 dilution (1.94 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the megakaryocytes in the human bone marrow, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183345 for 10 mins at room temperature. -
Immunohistochemistry (Frozen) analysis of mouse spleen tissue section labeling CD42b with purified ab183345 at 1/100 (1.9 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometry analysis of mouse splenocytes cell labeling CD42b with purified ab183345 at 1/20 dilution (9.7 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabeled cells (blue).
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Immunohistochemical analysis of paraffin embedded human spleen tissue labeling CD42b with ab183345 at 1/100 dilution.
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Flow cytometry analysis of HEL (human bone marrow erythroleukemia) labeling CD42b with purified ab183345 at 1/20 dilution (9.7 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabeled cells (blue).
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Flow cytometric analysis of rabbit anti-CD42b (SP219) antibody using ab183345 (diluted 1/400) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).
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Immunohistochemical analysis of paraffin embedded human bone marrow tissue labeling CD42b with ab183345 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab183345 は 8 報の論文で使用されています。
- Park SY et al. Direct Contact with Platelets Induces Podoplanin Expression and Invasion in Human Oral Squamous Cell Carcinoma Cells. Biomol Ther (Seoul) 30:284-290 (2022). PubMed: 35110423
- Li Y et al. PD-L1 Regulates Platelet Activation and Thrombosis via Caspase-3/GSDME Pathway. Front Pharmacol 13:921414 (2022). PubMed: 35784685
- Arnold S et al. Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine Tissues. Methods Protoc 5:N/A (2022). PubMed: 36136817
- Nauen DW et al. Assessing Brain Capillaries in Coronavirus Disease 2019. JAMA Neurol 78:760-762 (2021). PubMed: 33576767
- Essig F et al. High Mobility Group Box 1 Protein in Cerebral Thromboemboli. Int J Mol Sci 22:N/A (2021). PubMed: 34681935
- Maeda K et al. Hepatomegaly Associated with Non-Obstructive Sinusoidal Dilation in Experimental Visceral Leishmaniasis. Pathogens 10:N/A (2021). PubMed: 34832512
- Siddique SM et al. PKN1 kinase-negative knock-in mice develop splenomegaly and leukopenia at advanced age without obvious autoimmune-like phenotypes. Sci Rep 9:13977 (2019). PubMed: 31562379
- Guerrero A et al. Cardiac glycosides are broad-spectrum senolytics. Nat Metab 1:1074-1088 (2019). PubMed: 31799499