The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 25065646
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
platelet glycoprotein IIb of IIb/IIIa complex antibody
Platelet membrane glycoprotein IIb antibody
platelet specific antigen BAK antibody
Immunohistochemistry (Frozen sections) - Anti-CD41 antibody [M148] (ab11024)This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury (University of Cambridge, United Kingdom)
IHC-Fr image of C41 staining on human blood film using ab11024 (1:1000). The sample was fixed using paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The slides were then blocked with 10% Donkey Serum for 1 hr at 24°C. ab11024 was diluted 1:100 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and slides were incubated with the primary antibody for 4 hours at 24°C. The secondary antibody used was Donkey Polyclonal to rabbit IgG conjugated to Alexa Fuor 568 (1:1000)
Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [M148] (ab11024)This image was submitted by an anonymous abreviewer.
ICC/IF image of ab11024 staining of human platelet cells. The sections were incubated in 10% serum to block non-specific protein-protein interactions and in 0.2% triton X to permeabilise the cells. The sections were then incubated with ab11024 (1:400) for one hour at 20°C, followed by alexa 488 conjugated secondary antibody (green). Platelets were counterstained with rhodamine phalloidin to outline the cellular cytoskeleton (red).
Overlay histogram showing HL60 cells stained with ab11024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11024, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Rambøl MH et al. Recellularization of Decellularized Venous Grafts Using Peripheral Blood: A Critical Evaluation. EBioMedicine32:215-222 (2018).
Read more (PubMed: 29779699) »
Finnegan S et al. Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease. Arthritis Res Ther16:R8 (2014).
Read more (PubMed: 24410838) »