Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate (0.01M) pH=6
Permeabilization
Yes - 0.05% Tween
Specification
Brain tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s)
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: HRP + DAB
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Staining seemed very aspecific. Problem: CD40 should be expressed on the glial cells of the brain. However, the CD40 seems to stain the lipofuscin which accumulates in the brain with age.
Abcam response
Our Scientific Support team investigated in this negative Abreview, and we have resolved the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Boyd Kenkhuis
Verified customer
投稿 Aug 22 2018