Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain Cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ph6
Permeabilization
Yes - 0.1%
Specification
Brain Cortex
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Validated in cortex of individuals with CTE and glioblastoma. Will pick up some background when co-labeled with other markers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 03 2021