Anti-CD39 抗体 [EPR3678(2)] (ab108248)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3678(2)] to CD39
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD39 antibody [EPR3678(2)]
CD39 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3678(2)] to CD39 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human placenta, rat hippocampus, rat pancreas, mouse placenta and mouse urinary bladder tissue lysates; IM-9 and HUVEC cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3678(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108248の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
ターゲット情報
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機能
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well. -
組織特異性
Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. -
配列類似性
Belongs to the GDA1/CD39 NTPase family. -
翻訳後修飾
The N-terminus is blocked.
Palmitoylated in the N-terminal part. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 953 Human
- Entrez Gene: 12495 Mouse
- Entrez Gene: 64519 Rat
- Omim: 601752 Human
- SwissProt: P49961 Human
- SwissProt: P55772 Mouse
- SwissProt: P97687 Rat
- Unigene: 576612 Human
see all -
製品の状態
There are 3 isoforms produced by alternative splicing. -
別名
- ATPDase antibody
- CD 39 antibody
- CD39 antibody
see all
画像
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All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)
Lane 1 : Rat hippocampus tissue lysate
Lane 2 : Rat pancreas tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 70,74 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration 5% NFDM/TBST.
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All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)
Lane 1 : Mouse placenta tissue lysate
Lane 2 : Mouse urinary bladder tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer and concentration 5% NFDM/TBST.
The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.
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Lane 1 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/100 dilution (purified)
Lane 2 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)
All lanes : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 70,74 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration 5% NFDM/TBST.
Ref: PMID: 18693757
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All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (unpurified)
Lane 1 : Human placenta lysate
Lane 2 : IM-9 cell lysate
Predicted band size: 58 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab108248 は 17 報の論文で使用されています。
- Zhou X et al. Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice. Eur J Pain 26:991-1005 (2022). PubMed: 35138669
- Joung J et al. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat Commun 13:1606 (2022). PubMed: 35338135
- Calame DG et al. Biallelic Variants in the Ectonucleotidase ENTPD1 Cause a Complex Neurodevelopmental Disorder with Intellectual Disability, Distinct White Matter Abnormalities, and Spastic Paraplegia. Ann Neurol 92:304-321 (2022). PubMed: 35471564
- Macedo C et al. Donor-derived regulatory dendritic cell infusion results in host cell cross-dressing and T cell subset changes in prospective living donor liver transplant recipients. Am J Transplant 21:2372-2386 (2021). PubMed: 33171019
- Hamed FN et al. Alopecia areata patients show deficiency of FOXP3+CD39+ T regulatory cells and clonotypic restriction of Treg TCRß-chain, which highlights the immunopathological aspect of the disease. PLoS One 14:e0210308 (2019). PubMed: 31277078