Anti-CD38 抗体 [FS02] (ab1260)

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製品の概要

  • 製品名

    Anti-CD38 antibody [FS02]
    CD38 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [FS02] to CD38

  • 由来種

    Mouse

  • 特異性

    This antibody reacts with a type II membrane glycopeptide with a transmembrane sequence near the NH2-terminus.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報
    HIV Syndromes and other clinical situations characterized by lymphocyte in vivoactivation in human lysed whole peripheral blood or mononuclear cells separated by density gradient.

    CD38 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD38 is sufficient for labelling of 1x106 cells.

    • ターゲット情報

    • 機能

      Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.

    • 組織特異性

      Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.

    • 配列類似性

      Belongs to the ADP-ribosyl cyclase family.

    • 発生段階

      Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 製品の状態

        There are 2 isoforms produced by alternative splicing.

      • 別名

        • Acute lymphoblastic leukemia cells antigen CD38 antibody
        • ADP ribosyl cyclase 1 antibody
        • ADP ribosyl cyclase antibody
        • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
        • ADP-ribosyl cyclase 1 antibody
        • ADPRC 1 antibody
        • ADPRC1 antibody
        • cADPr hydrolase 1 antibody
        • CD 38 antibody
        • CD38 antibody
        • CD38 antigen (p45) antibody
        • CD38 antigen antibody
        • CD38 molecule antibody
        • Cd38-rs1 antibody
        • CD38_HUMAN antibody
        • CD38H antibody
        • Cyclic ADP ribose hydrolase antibody
        • Cyclic ADP ribose hydrolase 1 antibody
        • Cyclic ADP-ribose hydrolase 1 antibody
        • EC 3.2.2.5 antibody
        • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
        • I-19 antibody
        • I19 (mouse) antibody
        • Lymphocyte differentiation antigen CD38 antibody
        • NAD(+) nucleosidase antibody
        • NIM-R5 antigen antibody
        • NIMR5 antigen (mouse) antibody
        • OTTHUMP00000158633 antibody
        • OTTHUMP00000217743 antibody
        • p45 antibody
        • T10 antibody
        see all

      プロトコール

      参考文献

      ab1260 has not yet been referenced specifically in any publications.

      ab1260 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

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      1-3 of 3 Abreviews or Q&A

      Question

      Is this antibody[FS02] (ab1260) the agonistic IB4 clone? The answer provided does not clearly say Yes or No.

      The references given do not cite Abcam as the source of the antibody.

      Would you be able to clarify if this antibody indeed is or is not the IB4 agonistic antibody towards CD38?

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      Answer


      I can confirm that ab1260 is not the agonistic monoclonal antibody (IB4) stated in the literature and unfortunately, we do not have this product in the catalogue. ab1260 has the clone number FS02 instead of IB4 and we have no information regarding whether it is an agonistic monoclonal antibody.

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      Question

      Good morning,

      I wish to have specific information for the monoclonal antibodies of human CD38 available on your web site. More specifically, I would like to know the nature of the immunogen used to raise those monoclonal antibodies (e.g. amino acid sequence, or CD38 fragment). In other words, I am trying to identify the region of the CD38 protein where these antibodies bind.

      The following catalogue # are the monoclonal antibodies for CD38 available from your company:

      ab2577
      ab93941
      ab108403
      ab49644
      ab1260
      ab34283
      ab75231
      ab91390
      ab9504

      I truly hope you will be able to assist me or orient me toward someone else who could help answering my questions.

      Read More
      Answer

      Thank you for your enquiry.

      Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

      ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information the epitope mapping was not done. Only its location to the extracellular portion of the protein is known.

      ab93941: Purified recombinant fragment of Human CD38 expressed in E. coli.The peptide maps between amino acids 171 and 292.

      ab108403: A synthetic peptide corresponding to residues on the C terminus of Human CD38. The peptide maps between amino acids 275 and 300, on the C-terminus of human CD38 (UniProt: P28907).

      ab49644: Recombinant fusion protein corresponding to the extracellular region of human CD38 molecule. Unfortunately, futher details of this immunogen sequence are not available.

      ab1260: I am sorry but we do know the immunogen sequence or the range of amino acids or the region of the peptide from which it is derived. The producer of the hybridoma did not give us this kind of information.

      ab34283: The details of the immunogen for this antibody are also not available.

      ab75231: The antibody, CD 38 was raised to a prokaryotic recombinant protein corresponding to the extracellular region of the CD38 molecule. This region corresponds to amino acids 45-300. Unfortunately, the antibody has not been epitope mapped in order to determine the exact amino acid sequence to which it binds.

      ab91390: This monoclonal was development using as immunogen whole cell of human acute lymphoblastic B leukaemia. The antibody has also not been epitope mapped.

      ab9504: Nawalma cell line. The antibody has also not been epitope mapped.

      We aim to provide as much information as possible to customers, so I am sorry that this has not entirely been possible on this occasion. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information, or if we could be of any help in choosing the most suitable antibody for your experimental layout.

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      Question

      Is this antibody the agonistic IB4 clone referenced? If not, do you have one available. Thank you.

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      Answer

      Thank you for your email. Ab1260 has been used in the following two publications, please refer to them for more information regarding this antibody: Morra M et al. CD38 is functionally dependent on the TCR/CD3 complex in human T cells. FASEB J 12:581-92 (1998). PubMed: 9576485 Zupo S et al. CD38 signaling by agonistic monoclonal antibody prevents apoptosis of human germinal center B cells. Eur J Immunol 24:1218-22 (1994). PubMed: 8181532

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