Anti-CD38 抗体 [EPR4106] (ab108403)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4106] to CD38
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Biotin Carrier Free

製品名

Anti-CD38 antibody [EPR4106]
CD38 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR4106] to CD38
由来種

Rabbit
特異性

Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.
アプリケーション

適用あり: WB, IHC-P, Flow Cytmore details
適用なし: ICC/IF
種交差性

交差種: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Raji and Ramos cell lysates, Human lymph node lysate. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues. Flow Cyt: Raji cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
解離定数（K_D 値）

K_D = 1.40 x 10 ^-11 M
Learn more about K_D
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR4106
アイソタイプ

IgG
研究分野

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab108403の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/10000.
IHC-P		1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

特記事項
WB 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/10000.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

追加情報

Is unsuitable for ICC/IF.

機能

Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
組織特異性

Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
配列類似性

Belongs to the ADP-ribosyl cyclase family.
発生段階

Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
細胞内局在

Membrane.
Target information above from: UniProt accession P28907 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
製品の状態

There are 2 isoforms produced by alternative splicing.
別名
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
- ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
- ADP-ribosyl cyclase 1 antibody
- ADPRC 1 antibody
- ADPRC1 antibody
- cADPr hydrolase 1 antibody
- CD 38 antibody
- CD38 antibody
- CD38 antigen (p45) antibody
- CD38 antigen antibody
- CD38 molecule antibody
- Cd38-rs1 antibody
- CD38_HUMAN antibody
- CD38H antibody
- Cyclic ADP ribose hydrolase antibody
- Cyclic ADP ribose hydrolase 1 antibody
- Cyclic ADP-ribose hydrolase 1 antibody
- EC 3.2.2.5 antibody
- Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
- I-19 antibody
- I19 (mouse) antibody
- Lymphocyte differentiation antigen CD38 antibody
- NAD(+) nucleosidase antibody
- NIM-R5 antigen antibody
- NIMR5 antigen (mouse) antibody
- OTTHUMP00000158633 antibody
- OTTHUMP00000217743 antibody
- p45 antibody
- T10 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)

Lane 1 : Anti-CD38 antibody [EPR4106] (ab108403) at 1/200 dilution
Lane 2 : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution

Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 34 kDa

Buffer and concentration: 5% NFDM/TBST

Exposure time, Lane 1: 20 seconds, Lane 2: 30 seconds
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)

Anti-CD38 antibody [EPR4106] (ab108403) at 1/3000 dilution + Human lymph node lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 34 kDa

Exposure time: 80 seconds

Buffer and concentration: 5% NFDM/TBST
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)

All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution (purified)

Lane 1 : HuT-78 cell lysate
Lane 2 : Human fetal thymus tissue lysate
Lane 3 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-CD38 antibody [EPR4106] (ab108403)

Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CD38 with ab108403 at 1/900 dilution (1.03 µg/ml) (Red). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CD38 antibody [EPR4106] (ab108403)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

Tissue Microarrays stained for "Anti-CD38 antibody [EPR4106]” using "ab108403" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab108403 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Anti-CD38 antibody [EPR4106] (ab108403)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab108403 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108403 は 13 報の論文で使用されています。

Moribe F et al. Epigenetic suppression of SLFN11 in germinal center B-cells during B-cell development. PLoS One 16:e0237554 (2021). PubMed: 33513156
Zheng XZ et al. Elevation of erythrocyte sedimentation rate and C-reactive protein levels reflects renal interstitial inflammation in drug-induced acute tubulointerstitial nephritis. BMC Nephrol 21:514 (2020). PubMed: 33243164
Wang YX et al. Curative effect of methotrexate combined with teniposide in the treatment of primary central nervous system lymphoma. Oncol Lett 19:2097-2106 (2020). PubMed: 32194707
Damond N et al. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry. Cell Metab 29:755-768.e5 (2019). PubMed: 30713109
Huang YH et al. Decoration of Anti-CD38 on Nanoparticles Carrying a STAT3 Inhibitor Can Improve the Therapeutic Efficacy Against Myeloma. Cancers (Basel) 11:N/A (2019). PubMed: 30791634

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

Question

Good morning,

I wish to have specific information for the monoclonal antibodies of human CD38 available on your web site. More specifically, I would like to know the nature of the immunogen used to raise those monoclonal antibodies (e.g. amino acid sequence, or CD38 fragment). In other words, I am trying to identify the region of the CD38 protein where these antibodies bind.

The following catalogue # are the monoclonal antibodies for CD38 available from your company:

ab2577
ab93941
ab108403
ab49644
ab1260
ab34283
ab75231
ab91390
ab9504

I truly hope you will be able to assist me or orient me toward someone else who could help answering my questions.

Abcam community

Verified customer

Asked on Mar 12 2012

Answer

Thank you for your enquiry.

Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information the epitope mapping was not done. Only its location to the extracellular portion of the protein is known.

ab93941: Purified recombinant fragment of Human CD38 expressed in E. coli.The peptide maps between amino acids 171 and 292.

ab108403: A synthetic peptide corresponding to residues on the C terminus of Human CD38. The peptide maps between amino acids 275 and 300, on the C-terminus of human CD38 (UniProt: P28907).

ab49644: Recombinant fusion protein corresponding to the extracellular region of human CD38 molecule. Unfortunately, futher details of this immunogen sequence are not available.

ab1260: I am sorry but we do know the immunogen sequence or the range of amino acids or the region of the peptide from which it is derived. The producer of the hybridoma did not give us this kind of information.

ab34283: The details of the immunogen for this antibody are also not available.

ab75231: The antibody, CD 38 was raised to a prokaryotic recombinant protein corresponding to the extracellular region of the CD38 molecule. This region corresponds to amino acids 45-300. Unfortunately, the antibody has not been epitope mapped in order to determine the exact amino acid sequence to which it binds.

ab91390: This monoclonal was development using as immunogen whole cell of human acute lymphoblastic B leukaemia. The antibody has also not been epitope mapped.

ab9504: Nawalma cell line. The antibody has also not been epitope mapped.

We aim to provide as much information as possible to customers, so I am sorry that this has not entirely been possible on this occasion. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information, or if we could be of any help in choosing the most suitable antibody for your experimental layout.

Abcam Scientific Support

Answered on Mar 12 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

解離定数（KD 値）

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

発生段階

細胞内局在

参照データベース

製品の状態

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (13)

レビューと Q&A

解離定数（K_D 値）