Anti-CD38 抗体 [90] (ab61400)
Key features and details
- Rat monoclonal [90] to CD38
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-CD38 antibody [90]
CD38 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [90] to CD38 -
由来種
Rat -
アプリケーション
適用あり: Flow Cyt, IHC-Frmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD38. Mouse pre-B cells.
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ポジティブ・コントロール
- Flow Cyt: C57/BL6 splenocytes. IHC-Fr: Ms Spleen
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from TCS. -
一次抗体 備考
In mouse CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and not expressed by mature plasma cells. This pattern of expression contrasts strikingly with that seen in the human. -
ポリ/モノ
モノクローナル -
クローン名
90 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61400の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 1 µg/ml.
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IHC-Fr |
Use a concentration of 1 µg/ml.
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特記事項 |
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Flow Cyt
Use a concentration of 1 µg/ml. |
IHC-Fr
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
組織特異性
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
配列類似性
Belongs to the ADP-ribosyl cyclase family. -
発生段階
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12494 Mouse
- SwissProt: P56528 Mouse
- Unigene: 249873 Mouse
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製品の状態
There are 2 isoforms produced by alternative splicing. -
別名
- Acute lymphoblastic leukemia cells antigen CD38 antibody
- ADP ribosyl cyclase 1 antibody
- ADP ribosyl cyclase antibody
see all
画像
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C57BL/6 mouse splenocytes stained with ab61400 (right) or Rat IgG2aΚ (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab61400) or Rat IgG2a Isotype (1x106 in 100µl at 1 µg/ml) (left) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°. The cells were simultaneously stained with CD19 APC conjugated antibody.
Acquisition of >50,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
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IHC image of CD38 staining in a section of frozen normal mouse Spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab61400 at 1µg/ml. The section was then incubated with ab150165 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue). The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab61400 は論文での使用が確認できていません。