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リコンビナントRabMAb

Anti-CD36 抗体 [EPR22509-40] (ab252922)

Submit a review Submit a question References (1)
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)
  • Anti-CD36 antibody [EPR22509-40] (ab252922)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22509-40] to CD36
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CD36 antibody [EPR22509-40]
    CD36 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22509-40] to CD36

  • 由来種

    Rabbit

  • 特異性

    CD36 expression is low in the normal brain (PMID: 27646002).

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cytmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: U937 whole cell lysate; rat spleen and fat tissue lysates; human lung and placenta tissue lysates. IHC-P: Human spleen and hepatocellular carcinoma tissue; rat stomach tissue. Flow cyt: THP-1 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab252922の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 70-110 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is recommend only for human and rat.
Flow Cyt
1/50.
特記事項
WB
1/1000. Detects a band of approximately 70-110 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is recommend only for human and rat.
Flow Cyt
1/50.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
    (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.

  • 関連疾患

    Platelet glycoprotein IV deficiency
    Coronary heart disease 7

  • 配列類似性

    Belongs to the CD36 family.

  • 翻訳後修飾

    N-glycosylated and O-glycosylated with a ratio of 2:1.
    Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).

  • 細胞内局在

    Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Adipocyte membrane protein antibody
      • BDPLT10 antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
      • CD36 antigen antibody
      • CD36 molecule (thrombospondin receptor) antibody
      • CD36 molecule antibody
      • CD36_HUMAN antibody
      • CHDS7 antibody
      • Cluster determinant 36 antibody
      • Collagen receptor, platelet antibody
      • FAT antibody
      • Fatty acid translocase antibody
      • Fatty acid transport protein antibody
      • Glycoprotein IIIb antibody
      • GP IIIb antibody
      • GP3B antibody
      • GP4 antibody
      • GPIIIB antibody
      • GPIV antibody
      • Leukocyte differentiation antigen CD36 antibody
      • MGC108510 antibody
      • MGC91634 antibody
      • PAS 4 protein antibody
      • PAS IV antibody
      • PAS-4 antibody
      • PASIV antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein 4 antibody
      • Platelet glycoprotein IV antibody
      • scarb3 antibody
      • Scavenger receptor class B member 3 antibody
      • SR-B3 antibody
      • SRB3 antibody
      • Thrombospondin receptor antibody
      see all

    画像

    • Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
      All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

      Lane 1 : Rat spleen lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

      Predicted band size: 53 kDa
      Observed band size: 70-110 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM /TBST

      CD36 expression is low in the normal brain (PMID:27646002)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

      Immunohistochemical analysis of [paraffin-embedded human spleen tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human spleen (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

      Flow cytometric analysis of  THP-1 (human monocytic leukemia cell line) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

      Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
      All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1000 cells

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human lung tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

      Predicted band size: 53 kDa


      Exposure time: 3 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247).

    • Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)
      All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

      Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
      Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 53 kDa
      Observed band size: 70-110 kDa why is the actual band size different from the predicted?


      Exposure time: 8 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

      Negative control: Ramos, Jurkat (PMID: 27956597,10744776)

      The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247, 8631821).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

      Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat stomach. (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

      Immunohistochemical analysis of paraffin-embedded human hepatocellular tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human hepatocellular carcinoma (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

      Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cell line labelling CD36 with ab252922 at 1/50 dilution (red) compared with Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

      Negative control: Ramos (PMID: 10744776).Gated on viable cells.

    • Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)
      Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

      Flow cytometric analysis of  Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

      Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

      Gated on viable cells.

      Negative control: Ramos (PMID: 10744776).

       

    • Anti-CD36 antibody [EPR22509-40] (ab252922)
      Anti-CD36 antibody [EPR22509-40] (ab252922)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab252922 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab252922 は 1 報の論文で使用されています。

    • Supruniuk E  et al. Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes. Int J Mol Sci 21:N/A (2020). PubMed: 33171690

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