Application
Flow Cytometry
Fixation
None, cells were used fresh
Permeabilization
No
Sample
Mouse Cell (Cells from bone marrow (C57b6 mice))
Specification
Cells from bone marrow (C57b6 mice)
Gating Strategy
Alive cells (DAPI-), blood cells exluded
Preparation
Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Other product details
Incubation time
30 minute(s) · Temperature: 20°C · Diluent: PBS, BSA 2%, EDTA 3mM
Dilution
1/15
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Immuno Research, anti-rat
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight 488
Dilution
1/1000
Additional data
Additional Notes
After incubation with CD34 antibody, cells were centrifuged (400g, 5min), rinced with PBS-BSA 2%, centrifuged (400g, 5min) then secondary antibody and DAPI were added 20min prior to count cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Francois Daubeuf
Verified customer
投稿 Feb 24 2015