Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse xenograft-FFPE)
Specification
mouse xenograft-FFPE
Fixative
Formaldehyde
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: mouse bloc
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-rat (for CD34)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
I ran the CD34 with a monoclonal Smooth Muscle Actin for a dual stain in fluorescence. Heat antigen retrieval was in citrate in a steamer for 30 minutes, followed by cooling for 30 minutes.
See image, the CD34 is in green, the SMA in red
See image, the CD34 is in green, the SMA in red
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 08 2006