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  1. Link

    cd34-antibody-mec-147-ab8158.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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Anti-CD34 抗体 [MEC 14.7] (ab8158)

  • Datasheet
  • SDS
Reviews (34)Q&A (40)References (192)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
  • Western blot - Anti-CD34 antibody [MEC 14.7] (ab8158)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)

Key features and details

  • Rat monoclonal [MEC 14.7] to CD34
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

PE

こちらの製品もご検討ください

タンパク質
Recombinant Human CD34 protein (ab126924)
二次抗体
Product image
Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
一次抗体
Product image
Anti-LYVE1 antibody [RM1008] - BSA and Azide free (ab282748)

関連製品

製品の概要

  • 製品名

    Anti-CD34 antibody [MEC 14.7]
    CD34 一次抗体 製品一覧
  • 製品の詳細

    Rat monoclonal [MEC 14.7] to CD34
  • 由来種

    Rat
  • 特異性

    Recognizes mouse CD34.

  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Mouse CD34. Specifically, the murine endothelioma cell line tEnd.

  • ポジティブ・コントロール

    • IHC-P: Mouse lung and brain tissue. WB: Mouse lung membrane tissue lysate.
  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    MEC 14.7
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    kappa
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Stem cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

関連製品

  • Alternative Versions

    • Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)
    • PE Anti-CD34 antibody [MEC 14.7] (ab23830)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Recombinant Protein

    • Recombinant Human CD34 protein (ab126924)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8158の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (22)
Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Staining with this method can be difficult, it has been reported to us that milder fixation methods for paraffin sections like using zinc solution work well (unfortunately we have no further detailed instructions of this fixation method).

WB (1)
Use a concentration of 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 41 kDa).
特記事項
IHC-P
Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Staining with this method can be difficult, it has been reported to us that milder fixation methods for paraffin sections like using zinc solution work well (unfortunately we have no further detailed instructions of this fixation method).

WB
Use a concentration of 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 組織特異性

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 配列類似性

    Belongs to the CD34 family.
  • 発生段階

    On early hematopoietic progenitor cells.
  • 翻訳後修飾

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession P28906 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 12490 Mouse
    • SwissProt: Q64314 Mouse
    • Unigene: 29798 Mouse
    • 別名

      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • cluster of differentiation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)

      IHC image of CD34 staining in a formalin fixed paraffin embedded mouse lung tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    • Western blot - Anti-CD34 antibody [MEC 14.7] (ab8158)
      Western blot - Anti-CD34 antibody [MEC 14.7] (ab8158)
      Anti-CD34 antibody [MEC 14.7] (ab8158) at 1 µg/ml + Mouse Lung Membrane Tissue Lysate (ab171830) at 50 µg

      Secondary
      Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/20000 dilution

      Performed under reducing conditions.

      Predicted band size: 41 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      The main band is as expected at 80 kDa since the target is heavily glycosylated and phosphorylated.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)

      IHC image of CD34 staining in a formalin fixed paraffin embedded mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)This image is courtesy of an anonymous Abreview
      Immunohistochemical analysis of murine lung tissue, staining CD34 with ab8158.

      Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 16 hours at 4°C. An undiluted HRP-conjugated horse anti-rat polyclonal IgG was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)This image is courtesy of an anonymous Abreview.

      ab8158 staining CD34 in mouse mammary gland tissue sections by Immunohistochemistry (IHC-P - paraffin-embedded sections). Tissue was fixed with methacarnoy and blocked with 4% BSA for 60 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 16 hours. A undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)This image is courtesy of an anonymous Abreview

      ab8158 staining mouse brain tissue sections by IHC-P.  Tissue sections were paraformaldehyde fixed and blocked with 0.5% Perkin-Elmer TNB Blocking Buffer for 30 minutes at 25°C.  The primary antibody was diluted 1/100 and incubated for 18 hours at 4°C.  An biotin conjugated goat anti-rat was used as the secondary

      Photomicrograph demonstrates CD34 in red and collagen type IV (ab19808) in blue in normal, adult brain vessels. Tissue was perfusion-fixed and cut into 15µm slide-mounted cryostat sections (i.e., lightly fixed, but not paraffin embedded).

      See Abreview

    プロトコール

    • Recommended IHC-P protocol with ab8158

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (192)

    ab8158 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8158 は 192 報の論文で使用されています。

    • Tanimura Y  et al. Long-term model of colitis-associated colorectal cancer suggests tumor spread mechanism and nature of cancer stem cells. Oncol Lett 21:7 (2021). PubMed: 33240413
    • Wang L  et al. Combination CTLA-4 immunoglobulin treatment and ultrasound microbubble-mediated exposure improve renal function in a rat model of diabetic nephropathy. Aging (Albany NY) 13:8524-8540 (2021). PubMed: 33714204
    • Mu H  et al. H3.3 K36M Mutation as a Clinical Diagnosis Method of Suspected Chondroblastoma Cases. Orthop Surg 13:616-622 (2021). PubMed: 33620145
    • Xin Y  et al. Establishment of a Jaw Fibrosarcoma Patient-Derived Xenograft and Evaluation of the Tumor Suppression Efficacy of Plumbagin Against Jaw Fibrosarcoma. Front Oncol 10:1479 (2020). PubMed: 32974176
    • Troncoso F  et al. Advantages in Wound Healing Process in Female Mice Require Upregulation A2A-Mediated Angiogenesis under the Stimulation of 17ß-Estradiol. Int J Mol Sci 21:N/A (2020). PubMed: 32998232
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 74 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [MEC 14.7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution (S1699), 20min at >=90C
    Permeabilization
    Yes - 0.2% Triton X-100 in phosphate buffered saline
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    10% Neutral Buffered Formalin
    Read More

    DR. Kevin Thorburn

    Verified customer

    投稿 Jul 05 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-CD34 antibody [MEC 14.7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [MEC 14.7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [MEC 14.7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [MEC 14.7]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (sarcoma tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
    Permeabilization
    No
    Specification
    sarcoma tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 21°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 24 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [MEC 14.7]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (melanoma cell line xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
    Permeabilization
    No
    Specification
    melanoma cell line xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 16 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD34 antibody [MEC 14.7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cytospined Bone marrow cells)
    Permeabilization
    No
    Specification
    Cytospined Bone marrow cells
    Fixative
    Methanol
    Read More

    Mr. Francois Daubeuf

    Verified customer

    投稿 Apr 27 2015

    Flow Cytometry abreview for Anti-CD34 antibody [MEC 14.7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    None, cells were used fresh
    Permeabilization
    No
    Sample
    Mouse Cell (Cells from bone marrow (C57b6 mice))
    Specification
    Cells from bone marrow (C57b6 mice)
    Gating Strategy
    Alive cells (DAPI-), blood cells exluded
    Preparation
    Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
    Sample buffer: PBS-EDTA 3mM
    Read More

    Mr. Francois Daubeuf

    Verified customer

    投稿 Feb 24 2015

    Western blot abreview for Anti-CD34 antibody [MEC 14.7]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (lung)
    Specification
    lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 10 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody [MEC 14.7] - Hematopoietic Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate (pH 6)
    Sample
    Mouse Tissue sections (Metastatic Lung)
    Specification
    Metastatic Lung
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    DR. Victoria Bridgeman

    Verified customer

    投稿 Feb 05 2014

    1-10 of 74 Abreviews or Q&A

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