
Anti-CD34 抗体 [EP373Y] (ab81289)
製品の概要
-
製品名Anti-CD34 antibody [EP373Y]
CD34 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EP373Y] to CD34
-
由来種Rabbit
-
アプリケーション適用あり: WB, IHC-P, ICC/IF, IP, IHC-Fr, Flow Cytmore details
-
種交差性交差種: Mouse, Rat, Sheep, Dog, Human, African bush elephant
-
免疫原
Synthetic peptide within Human CD34 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P28906 -
ポジティブ・コントロール
- Western Blot control TF-1. IHC controls Human kidney, HUVEC cells and rat kidney. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain. IHC-P: Mouse prostate and kidney tissues. IHC-Fr: Mouse lung tissue.
-
特記事項
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
-
解離定数(KD 値)KD = 1.15 x 10 -10 M Learn more about KD
-
バッファーpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度Protein A purified
-
ポリ/モノモノクローナル
-
クローン名EP373Y
-
アイソタイプIgG
-
研究分野
関連製品
-
Alternative Products
- Anti-CD34 antibody [EPR2999] (ab110643)
- Anti-CD34 antibody [EP373Y] (Alexa Fluor® 488) (ab195013)
- Anti-CD34 antibody [EP373Y] (HRP) (ab195017)
- Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
- Anti-CD34 antibody [EPR19581-76] (ab222609)
- Anti-CD34 antibody [EP373Y] (Phycoerythrin) (ab223930)
- Anti-CD34 antibody [MEC 14.7] (ab8158)
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab81289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa). For unpurified use at 1/10000 - 1/100000. ab81289 is not suitable for rat and mouse species in WB application. |
|
IHC-P | 1/2500 - 1/5000. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
|
ICC/IF | 1/100 - 1/250.
|
|
IP | 1/30. | |
IHC-Fr | 1/100. | |
Flow Cyt | Use at an assay dependent concentration. |
ターゲット情報
-
機能Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
-
組織特異性Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
-
配列類似性Belongs to the CD34 family.
-
発生段階On early hematopoietic progenitor cells.
-
翻訳後修飾Highly glycosylated.
Phosphorylated on serine residues by PKC. -
細胞内局在Membrane.
- Information by UniProt
-
参照データベース
- Entrez Gene: 947 Human
- Entrez Gene: 12490 Mouse
- Entrez Gene: 305081 Rat
- Omim: 142230 Human
- SwissProt: P28906 Human
- SwissProt: Q64314 Mouse
- Unigene: 374990 Human
- Unigene: 29798 Mouse
see all -
別名
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.
-
Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.
Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
-
Flow Cytometry analysis of TF-1(human erythroleukemia ) cells labeling CD34 with purified ab81289 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
purified at 1/10000 dilution + TF-1 (Human bone marrow erythroleukemia cells) at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human angiosarcoma labeling CD34 with unpurified ab81289 at 1/100-1/250.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)This image is courtesy of an anonymous Abreview.Paraformaldehyde-fixed, paraffin-embedded mouse prostate tissue stained for CD34 using ab81289 at 1/150 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.
-
Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)This image is courtesy of an anonymous Abreview.Frozen mouse lung tissue stained for CD34 using ab81289 at 1/400 dilution in immunohistochemical analysis, followed by donkey anti-rabbit Alexa Fluor® 546 at 1/2000 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)This image is courtesy of an Abreview submitted by Mr. Rudolf Jung.Paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue stained for CD34 using ab81289 at 1/200 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal tonsil vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.
-
CD34 was immunoprecipitated from TF-1 (Human bone marrow erythroleukemia cells) cells using purified ab81289 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab81289. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST
-
Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)Image from Földes G et al, PLoS One. 2010 May 5;5(5):e10501, Fig 3.
Immunocytochemistry/Immunofluorescence analysis of human embryonic stem cell-derived endothelial cells labeling CD34 with unpurified ab81289 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% aTriton X-100. An Alexa Fluor® 647-conjugated secondary antibody was used at a 1/400 dilution. DAPI (blue) was used as the nuclear counter stain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal kidney vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal uterus vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)
IHC image of CD34 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab81289, 1/250 dilution, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
参考文献
This product has been referenced in:
- Muñoz NM et al. Comparison of dynamic contrast-enhanced magnetic resonance imaging and contrast-enhanced ultrasound for evaluation of the effects of sorafenib in a rat model of hepatocellular carcinoma. Magn Reson Imaging 57:156-164 (2019). Read more (PubMed: 30465870) »
- Guo H et al. MicroRNA-371a-3p promotes progression of gastric cancer by targeting TOB1. Cancer Lett 443:179-188 (2019). Read more (PubMed: 30529155) »