製品の概要

  • 製品名

    Anti-CD34 antibody [EP373Y]
    CD34 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EP373Y] to CD34
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IP, IHC-Fr, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Sheep, Dog, Human, African bush elephant
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide within Human CD34 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P28906

  • ポジティブ・コントロール

    • Western Blot control TF-1. IHC controls Human kidney, HUVEC cells and rat kidney. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain. IHC-P: Mouse prostate and kidney tissues. IHC-Fr: Mouse lung tissue.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa).

For unpurified use at 1/10000 - 1/100000.

ab81289 is not suitable for rat and mouse species in WB application. 

IHC-P 1/2500 - 1/5000.

See IHC antigen retrieval protocols.

For unpurified use at  1/100 - 1/250.

ICC/IF 1/100 - 1/250.

 

IP 1/30.
IHC-Fr 1/100.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 組織特異性

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 配列類似性

    Belongs to the CD34 family.
  • 発生段階

    On early hematopoietic progenitor cells.
  • 翻訳後修飾

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 細胞内局在

    Membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

  • Paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue stained for CD34 using ab81289 at 1/200 dilution in immunohistochemical analysis.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal kidney vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal uterus vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.


     

  • IHC image of CD34 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab81289, 1/250 dilution, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry analysis of TF-1(human erythroleukemia ) cells labeling CD34 with purified ab81289 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • purified at 1/10000 dilution + TF-1 (Human bone marrow erythroleukemia cells) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • CD34 was immunoprecipitated from TF-1 (Human bone marrow erythroleukemia cells) cells using purified ab81289 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab81289. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.

    Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Paraformaldehyde-fixed, paraffin-embedded mouse prostate tissue stained for CD34 using ab81289 at 1/150 dilution in immunohistochemical analysis.

    See Abreview

  • Frozen mouse lung tissue stained for CD34 using ab81289 at 1/400 dilution in immunohistochemical analysis, followed by donkey anti-rabbit Alexa Fluor® 546 at 1/2000 dilution.

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of human embryonic stem cell-derived endothelial cells labeling CD34 with unpurified ab81289 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% aTriton X-100. An Alexa Fluor® 647-conjugated secondary antibody was used at a 1/400 dilution. DAPI (blue) was used as the nuclear counter stain.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

参考文献

This product has been referenced in:

  • Hueso M  et al. An Exonic Switch Regulates Differential Accession of microRNAs to the Cd34 Transcript in Atherosclerosis Progression. Genes (Basel) 10:N/A (2019). Read more (PubMed: 30669689) »
  • Si Z  et al. Effects of thrombolysis within 6 hours on acute cerebral infarction in an improved rat embolic middle cerebral artery occlusion model for ischaemic stroke. J Cell Mol Med 23:2468-2474 (2019). Read more (PubMed: 30697923) »
See all 186 Publications for this product

レビューと Q&A

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1-10 of 33 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Liver
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

投稿 Jun 27 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: cell conditioner 1
Permeabilization
No
Specification
lung
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Apr 09 2019

Application
Flow Cytometry
Sample
Rat Cell (Peripheral Blood Mononuclear Cells)
Permeabilization
Yes - Perm Buffer
Gating Strategy
Live Lymphocyte/Monocyte Population
Specification
Peripheral Blood Mononuclear Cells
Preparation
Cell harvesting/tissue preparation method: Ficoll
Sample buffer: FACS Buffer
Fixation
Paraformaldehyde

Abcam user community

Verified customer

投稿 Mar 25 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate / pH6
Permeabilization
No
Specification
skin
Fixative
Formaldehyde

Dr. Ludovic Wrobel

Verified customer

投稿 Mar 11 2019

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate 10mM Tween 20 0.5%
Permeabilization
Yes - triton 0.2%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

投稿 Mar 08 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Kidney
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Oct 02 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (artery)
Permeabilization
No
Specification
artery
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Apr 30 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (liver in fetal pigs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate buffer
Permeabilization
Yes - 0,1% Saponin
Specification
liver in fetal pigs
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Feb 26 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jan 26 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone marrow after decalcification)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
Permeabilization
No
Specification
Bone marrow after decalcification
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Apr 27 2017

1-10 of 33 Abreviews

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