製品の概要
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製品名Anti-CD34 antibody [4H11(APG)]
CD34 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [4H11(APG)] to CD34
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由来種Mouse
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特異性This antibody reacts with Class III epitope on CD34 antigen.
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アプリケーション適用あり: IHC-P, WB, Flow Cytmore details
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種交差性交差種: Human
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免疫原
Tissue/ cell preparation (Human). (Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia).
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ポジティブ･コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度>95% by SDS-PAGE
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特記事項（精製）Purified from TCS
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ポリ/モノモノクローナル
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クローン名4H11(APG)
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アイソタイプIgG1
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 115 kDa. | |
| Flow Cyt | Use a concentration of 2 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
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組織特異性Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
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配列類似性Belongs to the CD34 family.
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発生段階On early hematopoietic progenitor cells.
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翻訳後修飾Highly glycosylated.
Phosphorylated on serine residues by PKC. -
細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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別名
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [4H11(APG)] (ab762)](https://www.abcam.co.jp/ps/datasheet/images/0/ab762/CD34-Primary-antibodies-ab762-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [4H11(APG)] (ab762)IHC image of CD34 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab762, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
参考文献
This product has been referenced in:
- Mattheij NJ et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). Read more (PubMed: 23519467) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"