Anti-CD33 抗体 [6C5/2] (ab11032)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)](https://www.abcam.co.jp/ps/products/11/ab11032/Images/ab11032-172917-anti-cd33-antibody-6c5-2-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)")
Key features and details
- Mouse monoclonal [6C5/2] to CD33
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-CD33 antibody [6C5/2]
CD33 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6C5/2] to CD33 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Fc-tag) corresponding to CD33.
-
ポジティブ･コントロール
- IHC-P: Human tonsil FFPE tissue sections
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
6C5/2 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11032の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (3) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). -
組織特異性
Monocytic/myeloid lineage cells. -
配列類似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻訳後修飾
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 945 Human
- Omim: 159590 Human
- SwissProt: P20138 Human
- Unigene: 83731 Human
-
別名
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)
IHC image of CD33 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11032, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab11032 は 6 報の論文で使用されています。
- Souza ACP et al. Short-Term High-Fat Diet Consumption Reduces Hypothalamic Expression of the Nicotinic Acetylcholine Receptor a7 Subunit (a7nAChR) and Affects the Anti-inflammatory Response in a Mouse Model of Sepsis. Front Immunol 10:565 (2019). PubMed: 30967878
- Liu J et al. Tumoral EHF predicts the efficacy of anti-PD1 therapy in pancreatic ductal adenocarcinoma. J Exp Med 216:656-673 (2019). PubMed: 30733283
- Zhao Y et al. Myeloid-Derived Suppressor Cells and ?dT17 Cells Contribute to the Development of Gastric MALT Lymphoma in H. felis-Infected Mice. Front Immunol 10:3104 (2019). PubMed: 32063899
- Calcinotto A et al. IL-23 secreted by myeloid cells drives castration-resistant prostate cancer. Nature 559:363-369 (2018). PubMed: 29950727
- Morton JJ et al. Dual use of hematopoietic and mesenchymal stem cells enhances engraftment and immune cell trafficking in an allogeneic humanized mouse model of head and neck cancer. Mol Carcinog 57:1651-1663 (2018). PubMed: 30129680
- Zou Z et al. Siglecs Facilitate HIV-1 Infection of Macrophages through Adhesion with Viral Sialic Acids. PLoS One 6:e24559 (2011). Flow Cyt . PubMed: 21931755