Anti-CD32B (phospho Y292) 抗体 [EP926Y] (ab68423)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP926Y] to CD32B (phospho Y292)
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD32B (phospho Y292) antibody [EP926Y]
CD32B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP926Y] to CD32B (phospho Y292) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IP: Raji whole cell lysate WB: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP926Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68423の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).
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IP |
1/20.
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特記事項 |
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WB
1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa). |
IP
1/20. |
ターゲット情報
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機能
Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis. -
組織特異性
Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. -
関連疾患
Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2213 Human
- Omim: 604590 Human
- SwissProt: P31994 Human
- Unigene: 654395 Human
- Unigene: 713617 Human
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別名
- CD32 antibody
- CD32 antigen antibody
- CD32B antibody
see all
画像
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ab68423 at 1/20 dilution immunoprecipitating CD32B in Raji (Human Burkitt's lymphoma) whole cell lysates.
Lane 1 (input): Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
Lane 2 (+): Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
For western blotting, ab68423 at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.Expsoure time: 1 second
Observed MW: 40-44 KDa
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All lanes : Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40,44 kDaExposure time: 5 seconds
Observed MW: 40-44 KDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab68423 は 3 報の論文で使用されています。
- Rieth N et al. Characterization of SM201, an anti-hFc?RIIB antibody not interfering with ligand binding that mediates immune complex dependent inhibition of B cells. Immunol Lett 160:145-50 (2014). PubMed: 24472604
- Sundgren NC et al. Coupling of fc? receptor I to fc? receptor IIb by SRC kinase mediates C-reactive protein impairment of endothelial function. Circ Res 109:1132-40 (2011). PubMed: 21940940
- Horton HM et al. Antibody-mediated coengagement of Fc?RIIb and B cell receptor complex suppresses humoral immunity in systemic lupus erythematosus. J Immunol 186:4223-33 (2011). PubMed: 21357255