Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E10.5 mouse embryos transverse section)
Specification
E10.5 mouse embryos transverse section
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step
NOVOCASTRA protein block as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 5% donkey serum + PBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rat 568 (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000
Additional data
Additional Notes
This is a transverse section of E10.5 mouse embryo. CD31 expresses in endothelium cells which locates in the surface of dorsal aorta and small vessels.
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投稿 May 13 2013