Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph Node)
Specification
Lymph Node
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3%
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: EnVision+, Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
- Deparaffinization and dehydration :
1. 2 times in xylene, 5 min each
2. 100% ethanol, 2 changes, 2 min each
3. 95%
4. 70%
5. 50%
6. 30%
7. PBS
- Heat-based antigen retrieval : water bath, 96°C, 40 min, citrate buffer (pH 6)
- Inactivation of endogenous peroxydase : 3% hydrogen peroxide in H2O, 10 min
- Block slides with 3% serum
- Incubation with primary antibody (overnight, 4°C)
- Incubation with secondary antibody (1h, room temperature)
- Revelation (AEC)
1. 2 times in xylene, 5 min each
2. 100% ethanol, 2 changes, 2 min each
3. 95%
4. 70%
5. 50%
6. 30%
7. PBS
- Heat-based antigen retrieval : water bath, 96°C, 40 min, citrate buffer (pH 6)
- Inactivation of endogenous peroxydase : 3% hydrogen peroxide in H2O, 10 min
- Block slides with 3% serum
- Incubation with primary antibody (overnight, 4°C)
- Incubation with secondary antibody (1h, room temperature)
- Revelation (AEC)
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投稿 May 14 2007