Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Pseudo colored Green immunofluorescent staining of paraffin embedded mouse kidney with purified ab9498 Anti-CD31 at a working dilution of 1 in 100. The nuclei are Blue after staining with Hoechst and a light Red counter stain was created with a methyl green staining protocol. The antigen retrieval was performed with the NxGen decloaking chamber at pH6.0 . Alexa Fluor 647 secondary is preferred for its better resolution and total lack of autofluorescence.
Abcam response
We would like to thank the reviewer for submitting this AbReview for ab9498 and for their valuable feedback. We have not yet tested this antibody on murine samples, we were unsure what the result might be, and we appreciate the time and effort that the reviewer has put into testing this antibody in this species.
We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Feb 11 2020