Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver, spleen, lung, kidney, heart)
Specification
Liver, spleen, lung, kidney, heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10
Other product details
Dilution
1/50
Incubation time
20 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Biotinylated anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Tissue was fixed in 10% neutral buffered formalin and paraffin embedded. Used Antigen Unmasking Solution (Vector Labs) equivalent to citrate buffer pH 6 for antigen retrieval. Did avidin/biotin blocking step to block endogenous biotin in kidney and liver tissue, the normal goat serum block and standard ABC protocol.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Kelly Hudkins-Loya
Verified customer
投稿 Aug 25 2006