製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to CD31
  • 由来種

    Rabbit
  • 特異性

    Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries.
  • アプリケーション

    適用あり: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr, WBmore details
  • 種交差性

    交差種: Mouse, Human, Pig
  • 免疫原

    Synthetic peptide within Mouse CD31 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q08481

  • ポジティブ・コントロール

    • IHC-P: Mouse skin, xenograft tissue; Human tonsil tissue. WB: Jurkat whole cell lysate (ab7899), Human vascular endothelial cell lysate. IHC-Fr: Human fetal heart tissue.
  • 特記事項

      

アプリケーション

Our Abpromise guarantee covers the use of ab28364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/20. PubMed: 17316380
IHC-FoFr Use at an assay dependent concentration.
WB 1/500.

ターゲット情報

  • 機能

    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 組織特異性

    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 配列類似性

    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメイン

    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻訳後修飾

    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 細胞内局在

    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

画像

  • CLARITY-based immunochemistry showing Nerve fibers and vessels in the mesentery.

    A) Catecholaminergic nerve fibers stained for TH. (B) Nerves stained for the pan-neuronal marker TUJ-1. (A,B) Illustrate the integration of nerve fibers in the mesentery (asterisk) into the intramural neuronal networks of the gut. (C) Depicts blood vessels stained with the endothelial marker CD31 (using ab28364 at 1:50) and shows the branching of vessels coming from the mesentery (asterisk) and fine capillaries in the villi (confocal image stack 10x/0.3 objective, z-range 1047 μm). (D–F) Show the innervation of blood vessels in the mesentery ((D) CD31 (using ab28364 at 1:50), (E) TUJ-1, (F) Merge). (G–I) Identifies the very fine sympathetic nerve fibers of arterioles by specific catecholaminergic staining. Scale bars: (A–C) 500 μm; (D–F) 100 μm; (G–I) 100 μm.

    Displaying the mesentery and intact gut in one preparation is a major improvement in visualizing the neuronal and vascular interface between the alimentary tract and its extrinsic supply, rendering CLARITY-based immunochemistry a promising tool going beyond previous whole mount preparations.

    D can be visualized  Supplementary video 6 to fully appreciate the three-dimensional tissue composition.

  • ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ab28364 at a 1/300 dilution, staining human CD31 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/50 dilution staining mouse tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was formaldehyde fixed and blocked with serum. The tissue was incubated with the primary antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary. The endothelium of vessels of any tissues are positive.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/500 dilution + Human vascular endothelial cells at 25 µg

    Secondary
    anti-rabbit HRP at 1/5000 dilution

    See Abreview

  • Representative images of cross sections with CD31 staining in red, nucleus in blue. White dotted lines indicate the graft wall. Scale bar = 100 μm.

    Explanted grafts from C57/BL6 mice were fixed overnight in paraformaldehyde (2%) at room temperature. Samples were dehydrated through an ethanol gradient and embedded in paraffin and sectioned at 5 μm transversely from proximal anastomosis to distal anastomosis. For immunohistochemistry staining, sections were deparaffinised and stained with antibody against CD31 (Abcam, ab28364, 1:200) for endothelial cells.

     

  • ab28364 staining CD31 in mouse xenograft tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices and blocked with 1% BSA for 60 minutes at 21°C. Antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. The sample was incubated with primary antibody (20 ug/mL) at 4°C for 16 hours. A HRP-conjugated goat anti-rabbit polyclonal (2 ug/mL) was used as the secondary antibody.

    See Abreview

  • GFP positive cells (green) were colocalized with CD31 (red). Nuclear staining with DAPI is blue.

    For immunofluorescence staining, the skin samples were fixed in 4% paraformaldehyde and embedded in paraffin. Skin sections were stained with an anti-GFP antibody. The skin sections were also treated with primary Abs against CD31 (Abcam, ab28364, diluted 1:300). Secondary antibodies conjugated to rhodamine-isothiocyanate were used for fluorescence detection on a confocal laser scanning fluorescence microscope (Zeiss, German).

    Opn-/-; OPN (Osteopontin) knock-out mice.

参考文献

This product has been referenced in:

  • Xu L  et al. ATM deficiency promotes progression of CRPC by enhancing Warburg effect. Endocr Relat Cancer 26:59-71 (2019). Read more (PubMed: 30400006) »
  • Gallina D & Lincoln J Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell Populations. Anat Rec (Hoboken) 302:108-116 (2019). Read more (PubMed: 30412364) »
See all 948 Publications for this product

レビューと Q&A

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1-10 of 124 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Jul 11 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

投稿 Jul 10 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.1 TE
Permeabilization
Yes - 3% triton
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Jun 11 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Tongue)
Permeabilization
No
Specification
Tongue
Blocking step
DAKO blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 24°C
Fixative
Acetone

Abcam user community

Verified customer

投稿 May 30 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (hemangiosarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
Yes - Tween-20
Specification
hemangiosarcoma
Blocking step
Leica IHC/ISH Super Blocking as blocking agent for 40 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
10% neutral buffered formalin

Abcam user community

Verified customer

投稿 May 28 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 8.5 80 C
Permeabilization
Yes - 0.3% triton X-100
Specification
Kidney
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
10%NBF

Abcam user community

Verified customer

投稿 May 02 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (B16F10 (melanoma) syngeneic mouse tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - 3% H2O2
Specification
B16F10 (melanoma) syngeneic mouse tumor
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Apr 30 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (liver, endothelial)
Permeabilization
No
Specification
liver, endothelial
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Miss. Kara Shumansky

Verified customer

投稿 Mar 11 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: cirtric acid
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Miss. Jin Young Park

Verified customer

投稿 Nov 12 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (skin)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Miss. Jin Young Park

Verified customer

投稿 Nov 12 2018

1-10 of 124 Abreviews

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