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    cd31-antibody-ab28364.pdf

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Anti-CD31 抗体 (ab28364)

  • Datasheet
  • SDS
Reviews (148)Q&A (31)References (1173)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)

    Key features and details

    • Rabbit polyclonal to CD31
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-CD31 antibody [EPR3094] (ab76533)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-CD31 antibody
      CD31 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to CD31
    • 由来種

      Rabbit
    • 特異性

      Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries.
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Pig
    • 免疫原

      Synthetic peptide within Mouse CD31 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
      Database link: Q08481

    • 特記事項

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C.
    • バッファー

      pH: 7.60
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Cardiovascular
      • Blood
      • Platelets
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Cell Adhesion Molecules
      • Endothelial
      • Cardiovascular
      • Angiogenesis
      • Angiogenic Factors
      • Stem Cells
      • Mesenchymal Stem Cells
      • Surface Molecules
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Other
      • Cancer
      • Tumor immunology
      • CD markers
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Cell adhesion molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Thrombocytic Lineage
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Endothelial Cells
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers
      • Signal Transduction
      • Antibodies
      • cd31

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant human CD31 protein (ab155607)
    • Related Products

      • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
      • Mouse CD31 Matched Antibody Pair Kit (ab212065)
      • Anti-CD31 antibody [P2B1] (ab24590)
      • Anti-CD34 antibody [MEC 14.7] (ab8158)

    アプリケーション

    Our Abpromise guarantee covers the use of ab28364 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.

    Positive Control: Hu tonsil tissue

    ターゲット情報

    • 機能

      Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • 組織特異性

      Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • 配列類似性

      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • ドメイン

      The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • 翻訳後修飾

      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • 細胞内局在

      Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Target information above from: UniProt accession P16284 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 5175 Human
      • Entrez Gene: 18613 Mouse
      • Omim: 173445 Human
      • SwissProt: P16284 Human
      • SwissProt: Q08481 Mouse
      • Unigene: 376675 Human
      • Unigene: 514412 Human
      • Unigene: 343951 Mouse
      • 別名

        • Adhesion molecule antibody
        • CD31 antibody
        • CD31 antigen antibody
        • CD31 EndoCAM antibody
        • EndoCAM antibody
        • FLJ34100 antibody
        • FLJ58394 antibody
        • GPIIA antibody
        • GPIIA' antibody
        • PECA1 antibody
        • PECA1_HUMAN antibody
        • Pecam 1 antibody
        • PECAM 1 CD31 EndoCAM antibody
        • PECAM antibody
        • PECAM-1 antibody
        • Pecam1 antibody
        • Platelet and endothelial cell adhesion molecule 1 antibody
        • Platelet endothelial cell adhesion molecule antibody
        • Platelet/endothelial cell adhesion molecule 1 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)

        ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

      プロトコール

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
      • SDS
    • 参考文献 (1173)

      ab28364 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab28364 は 1173 報の論文で使用されています。

      • Stock AA  et al. Conformal Coating of Stem Cell-Derived Islets for ß Cell Replacement in Type 1 Diabetes. Stem Cell Reports 14:91-104 (2020). PubMed: 31839542
      • Barbeito-Andrés J  et al. Congenital Zika syndrome is associated with maternal protein malnutrition. Sci Adv 6:eaaw6284 (2020). PubMed: 31950075
      • Ayanlaja AA  et al. Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. Cell Commun Signal 18:24 (2020). PubMed: 32050972
      • Rutkai I  et al. Chronic imaging of mitochondria in the murine cerebral vasculature using in vivo two-photon microscopy. Am J Physiol Heart Circ Physiol 318:H1379-H1386 (2020). PubMed: 32330090
      • Luo S  et al. Targeting self-assembly peptide for inhibiting breast tumor progression and metastasis. Biomaterials 249:120055 (2020). PubMed: 32315863
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 179 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Tonsil)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: BioCare Diva decloaker
      Permeabilization
      No
      Specification
      Tonsil
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
      Fixative
      Formaldehyde
      Read More

      Mr. Victor Bernal

      Verified customer

      投稿 Nov 07 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (palatine tonsil)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA
      Permeabilization
      No
      Specification
      palatine tonsil
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Nov 02 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (spleen)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
      Permeabilization
      No
      Specification
      spleen
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Nov 02 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD31 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Pig Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Jun 22 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Mrs. Kyunga Kwak

      Verified customer

      投稿 May 07 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD31 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (Cerebellum)
      Antigen retrieval step
      None
      Permeabilization
      Yes - PBS 1x, Triton x-100 1%
      Specification
      Cerebellum
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Mar 19 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Tumour, Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate
      Permeabilization
      Yes - TBST
      Specification
      Tumour, Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More

      Stephen Turnock

      Verified customer

      投稿 Mar 09 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD31 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 28 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD31 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 28 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Pig Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 14 2020

      1-10 of 179 Abreviews or Q&A

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      For licensing inquiries, please contact partnerships@abcam.com

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