Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5226] to CD300a - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
製品名Anti-CD300a antibody [EPR5226] - BSA and Azide free
CD300a 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5226] to CD300a - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: ICC,IHC-P or IP
Synthetic peptide within Human CD300a aa 50-150. The exact sequence is proprietary.
ab248014 is the carrier-free version of ab124810 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248014 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as CD300 antigen
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 3.90 x 10 -12 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab248014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa).|
機能Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation.
組織特異性Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.
配列類似性Belongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
翻訳後修飾Phosphorylated on tyrosine.
- Information by UniProt
- CD300 antigen-like family member A antibody
- CD300a antibody
- CD300a antigen antibody
All lanes : Anti-CD300a antibody [EPR5226] (ab124810) at 1/1000 dilution
Lane 1 : Human lymph node lysates
Lane 2 : Human spleen lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
This data was developed using ab124810, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.