Anti-CD30 抗体 [BLR055F] - BSA free (ab272089)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR055F] to CD30 - BSA and Azide free
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD30 antibody [BLR055F] - BSA free
CD30 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [BLR055F] to CD30 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-P, ICCmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human CD30 aa 1-400. NP_001234.3. Extracellular domain.
Database link: P28908 -
ポジティブ・コントロール
- IP: MJ whole cell lysate. IHC-P: Human tonsil tissue. ICC: SR cells.
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特記事項
ab272089 is the BSA-free version of ab252561.
This product is sold under License from Bethyl Laboratories, Inc.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
キャリア・フリー
はい -
Concentration information loading...
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特記事項(精製)
Purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
BLR055F -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272089の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. -
配列類似性
Contains 6 TNFR-Cys repeats. -
翻訳後修飾
Phosphorylated on serine and tyrosine residues. -
細胞内局在
Cytoplasm and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 943 Human
- Omim: 153243 Human
- SwissProt: P28908 Human
- Unigene: 1314 Human
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別名
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
see all
画像
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Formalin-fixed, paraffin-embedded sections of SR (human large cell immunoblastic lymphoma cell line) cells stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).
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CD30 was immunoprecipitated from MJ (human cutaneous T cell lymphoma cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252561 at 5 µL per reaction. Western blot was performed from the immunoprecipitate using ab252561 at 1/1000 dilution.
Lane 1: ab252561 IP in MJ whole cell lysate.
Lane 2: Control IgG IP in MJ whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272089 は論文での使用が確認できていません。